PXD007596 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MHC peptides presentation is limited by availability of empty HLA molecules rather than the supply of peptides ligands |
Description | Whether the presentation levels of the major histocompatibility complex (MHC, called the human leukocytes antigens, HLA, in humans) is limited by the availability of peptide ligands for loading or by the supply of empty MHC molecules, is a yet unresolved issue. This study aims to tackle this dilemma using large-scale immunopeptidome analyses. First, cultured cells (MCF-7) were treated with interferons, which dramatically increased presentation levels of their HLA-B molecules, much more than HLA-A and HLA-C. The differential increase in the HLA-B molecules with their bound peptides, was driven by elevated HLA synthesis levels and not by supply of peptides, since all three HLA allotypes draw peptides from the same peptide pool, which should have been affected similarly by the interferons treatment. In addition, artificial competition for peptides was created by recombinant high levels expression of soluble HLA-A*02:01 molecules, in the same MCF-7 cells and on top of their endogenous membranal HLA-A*02:01. This high level expression of the soluble HLA did not affect the endogenous membranal HLA-A*02:01 peptidomes or its cell surface presentation levels. We therefore concluded that a surplus supply of peptides is available for loading and that presentation levels are more likely limited by the supply of HLA molecules. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:01:44.198.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dganit Melamed Kadosh |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-08-30 01:11:15 | ID requested | |
1 | 2018-05-10 03:03:45 | announced | |
⏵ 2 | 2024-10-22 04:01:50 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1002/pmic.201700248; |
Komov L, Kadosh DM, Barnea E, Milner E, Hendler A, Admon A, Cell Surface MHC Class I Expression Is Limited by the Availability of Peptide-Receptive "Empty" Molecules Rather than by the Supply of Peptide Ligands. Proteomics, 18(12):e1700248(2018) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Interferon, MHC, Peptidome, Immunopeptidome,HLA |
Contact List
Arie Admon |
contact affiliation | Department of Biology, Technion-Israel Institute of Technology |
contact email | admon@technion.ac.il |
lab head | |
Dganit Melamed Kadosh |
contact affiliation | Technion |
contact email | sdganit@tx.technion.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007596
- Label: PRIDE project
- Name: MHC peptides presentation is limited by availability of empty HLA molecules rather than the supply of peptides ligands