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PXD007596

PXD007596 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMHC peptides presentation is limited by availability of empty HLA molecules rather than the supply of peptides ligands
DescriptionWhether the presentation levels of the major histocompatibility complex (MHC, called the human leukocytes antigens, HLA, in humans) is limited by the availability of peptide ligands for loading or by the supply of empty MHC molecules, is a yet unresolved issue. This study aims to tackle this dilemma using large-scale immunopeptidome analyses. First, cultured cells (MCF-7) were treated with interferons, which dramatically increased presentation levels of their HLA-B molecules, much more than HLA-A and HLA-C. The differential increase in the HLA-B molecules with their bound peptides, was driven by elevated HLA synthesis levels and not by supply of peptides, since all three HLA allotypes draw peptides from the same peptide pool, which should have been affected similarly by the interferons treatment. In addition, artificial competition for peptides was created by recombinant high levels expression of soluble HLA-A*02:01 molecules, in the same MCF-7 cells and on top of their endogenous membranal HLA-A*02:01. This high level expression of the soluble HLA did not affect the endogenous membranal HLA-A*02:01 peptidomes or its cell surface presentation levels. We therefore concluded that a surplus supply of peptides is available for loading and that presentation levels are more likely limited by the supply of HLA molecules.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:01:44.198.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDganit Melamed Kadosh
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive; LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-08-30 01:11:15ID requested
12018-05-10 03:03:45announced
22024-10-22 04:01:50announced2024-10-22: Updated project metadata.
Publication List
10.1002/pmic.201700248;
Komov L, Kadosh DM, Barnea E, Milner E, Hendler A, Admon A, Cell Surface MHC Class I Expression Is Limited by the Availability of Peptide-Receptive "Empty" Molecules Rather than by the Supply of Peptide Ligands. Proteomics, 18(12):e1700248(2018) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Interferon, MHC, Peptidome, Immunopeptidome,HLA
Contact List
Arie Admon
contact affiliationDepartment of Biology, Technion-Israel Institute of Technology
contact emailadmon@technion.ac.il
lab head
Dganit Melamed Kadosh
contact affiliationTechnion
contact emailsdganit@tx.technion.ac.il
dataset submitter
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Dataset FTP location
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