PXD007590

PXD007590 is an original dataset announced via ProteomeXchange.

Title	Characterization of Spp1-TAP and Mer2-TAP interactomes
Description	Histone H3K4 methylation is a feature of meiotic recombination hotspots shared by many organisms including plants and mammals. Meiotic recombination is initiated by programmed double strand break (DSB) formation that in budding yeast is directed in gene promoters by histone H3K4 di/trimethylation. This histone modification is indeed recognized by Spp1, a PHD-finger containing protein that belongs to the conserved histone H3K4 methyltransferase Set1 complex. During meiosis, Spp1 binds H3K4me and recruits a DSB protein, Mer2, to promote DSB formation close to gene promoters. How Set1C and Mer2 related functions of Spp1 are connected is not clear.
HostingRepository	PRIDE
AnnounceDate	2018-01-30
AnnouncementXML	Submission_2018-02-23_04:20:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-29 07:45:01	ID requested
1	2018-01-30 08:34:23	announced
⏵ 2	2018-02-23 04:20:38	announced	Updated publication reference for PubMed record(s): 29444071.

Adam C, Gu, é, rois R, Citarella A, Verardi L, Adolphe F, B, é, neut C, Sommermeyer V, Ramus C, Govin J, Cout, é Y, Borde V, The PHD finger protein Spp1 has distinct functions in the Set1 and the meiotic DSB formation complexes. PLoS Genet, 14(2):e1007223(2018) [pubmed]

curator keyword: Biological

submitter keyword: Saccharomyces cerevisiae, meiosis, Spp1, Mer2, interactomics

Virginie Brun
contact affiliation	EDyP
contact email	virginie.brun@cea.fr
lab head
Yohann Couté
contact affiliation	Laboratoire d'Etude de la Dynamique des Protéomes
contact email	yohann.coute@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD007590

PRIDE project URI

• PRIDE
  1. PXD007590
     1. Label: PRIDE project
     2. Name: Characterization of Spp1-TAP and Mer2-TAP interactomes