PXD007588
PXD007588 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protein profile at different cellular fractions of Mycobacterium tuberculosis strains after exposure to Isoniazid |
| Description | Out of the 10 million of tuberculosis (TB) cases estimated in the world, around 14% are isoniazid (INH) resistant among new cases and 29% among previously treated cases in the last decade. INH is one of the oldest but also one of the more potent drugs to eliminate Mycobacterium tuberculosis (Mtb), the causing agent of TB. Because of the efficiency of isoniazid (INH) against Mycobacterium tuberculosis (Mtb), many studies are still focused in better understand its role in different bacterial metabolic pathways. We recently conducted a study that evaluated the changes in the protein abundance at different cellular fractions when clonal strains of Mtb developed INH resistance in the clinical and laboratory setting. Here, we want to establish which of the protein changes occurred or started because of the initial exposure to INH. Additionally, we wanted to evaluate if those changes happen differently in strains that are sensitive or resistant to INH, evaluating different cellular compartments from two different genetic lineages of Mtb. For this purpose, we analyzed the proteome of each cellular compartment (cytosol, cell wall, membrane and secreted proteins) through liquid chromatography (nano-HPLC) coupled to mass spectrometry using the Orbitrap Velos instrument and a t-test to perform the statistical analysis for each pair comparison. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_04:01:14.784.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD007588 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Luisa Nieto Ramirez |
| SpeciesList | scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2017-08-29 05:52:26 | ID requested | |
| 1 | 2019-06-17 04:33:10 | announced | |
| ⏵ 2 | 2024-10-22 04:01:15 | announced | 2024-10-22: Updated project metadata. |
Publication List
| 10.1016/j.dib.2019.103953; |
| Nieto Ramirez LM, Mehaffy C, Dobos KM, strains after exposure to isoniazid. Data Brief, 24():103953(2019) [pubmed] |
| 10.6019/PXD007588; |
Keyword List
| curator keyword: Biological |
| submitter keyword: cellular fractions,Tuberculosis, Isoniazid, shotgun proteomics, LC-MS/MS |
Contact List
| Karen M. Dobos, Ph.D. | |
|---|---|
| contact affiliation | Director, Research Integrity and Compliance Review Office Associate Professor, Mycobacteria Research Laboratories Dept of Microbiology, Immunology,& Pathology Colorado State University Ft. Collins, CO |
| contact email | karen.dobos@colostate.edu |
| lab head | |
| Luisa Nieto Ramirez | |
| contact affiliation | Colorado State University |
| contact email | nieto.luisa@gmail.com |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD007588 |
| PRIDE project URI |
Repository Record List
[ + ]
to receive all new ProteomeXchange dataset release announcements!
