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PXD007588

PXD007588 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein profile at different cellular fractions of Mycobacterium tuberculosis strains after exposure to Isoniazid
DescriptionOut of the 10 million of tuberculosis (TB) cases estimated in the world, around 14% are isoniazid (INH) resistant among new cases and 29% among previously treated cases in the last decade. INH is one of the oldest but also one of the more potent drugs to eliminate Mycobacterium tuberculosis (Mtb), the causing agent of TB. Because of the efficiency of isoniazid (INH) against Mycobacterium tuberculosis (Mtb), many studies are still focused in better understand its role in different bacterial metabolic pathways. We recently conducted a study that evaluated the changes in the protein abundance at different cellular fractions when clonal strains of Mtb developed INH resistance in the clinical and laboratory setting. Here, we want to establish which of the protein changes occurred or started because of the initial exposure to INH. Additionally, we wanted to evaluate if those changes happen differently in strains that are sensitive or resistant to INH, evaluating different cellular compartments from two different genetic lineages of Mtb. For this purpose, we analyzed the proteome of each cellular compartment (cytosol, cell wall, membrane and secreted proteins) through liquid chromatography (nano-HPLC) coupled to mass spectrometry using the Orbitrap Velos instrument and a t-test to perform the statistical analysis for each pair comparison.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:01:14.784.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD007588
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLuisa Nieto Ramirez
SpeciesList scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationListOxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-08-29 05:52:26ID requested
12019-06-17 04:33:10announced
22024-10-22 04:01:15announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.dib.2019.103953;
Nieto Ramirez LM, Mehaffy C, Dobos KM, strains after exposure to isoniazid. Data Brief, 24():103953(2019) [pubmed]
10.6019/PXD007588;
Keyword List
curator keyword: Biological
submitter keyword: cellular fractions,Tuberculosis, Isoniazid, shotgun proteomics, LC-MS/MS
Contact List
Karen M. Dobos, Ph.D.
contact affiliationDirector, Research Integrity and Compliance Review Office Associate Professor, Mycobacteria Research Laboratories Dept of Microbiology, Immunology,& Pathology Colorado State University Ft. Collins, CO
contact emailkaren.dobos@colostate.edu
lab head
Luisa Nieto Ramirez
contact affiliationColorado State University
contact emailnieto.luisa@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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