PXD007585

PXD007585 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	AP-MS analysis of murine and human norovirus VPg interactomes
Description	The norovirus VPg protein is covalently linked to the viral genome in place of a 5' cap, and functions as a cap-substitute, capable of interacting with translation initiation factors. Following on from our previous study (Chung et. al. 2014, J. BIol. Chem.) we wished to determine the interactome of human norovirus VPg, and compare that of murine norovirus VPg. We had previously demonstrated that mutation of the penultimate C-terminal phenylalanine residue in murine norovirus VPg greatly reduced initiation factor binding (F123A). Insertion of the equivalent mutation into human norovirus (F137A) also reduced initiation factor binding. Affinity purification of wild-type of mutant human and murine norovirus VPg was accomplished using GFP-tagged VPg transfected into SILAC-labelled human HEK-293T cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:01:26.985.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edward Emmott
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue
Instrument	LTQ Orbitrap Velos; Orbitrap Fusion ETD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-29 02:09:18	ID requested
1	2019-09-11 09:10:10	announced
⏵ 2	2024-10-22 04:01:27	announced	2024-10-22: Updated project metadata.

Publication List

10.7554/elife.46681;
Hosmillo M, Lu J, McAllaster MR, Eaglesham JB, Wang X, Emmott E, Domingues P, Chaudhry Y, Fitzmaurice TJ, Tung MK, Panas MD, McInerney G, Locker N, Wilen CB, Goodfellow IG, Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. Elife, 8():(2019) [pubmed]

10.7554/elife.46681;

Hosmillo M, Lu J, McAllaster MR, Eaglesham JB, Wang X, Emmott E, Domingues P, Chaudhry Y, Fitzmaurice TJ, Tung MK, Panas MD, McInerney G, Locker N, Wilen CB, Goodfellow IG, Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. Elife, 8():(2019) [pubmed]

Keyword List

curator keyword: Biomedical
submitter keyword: Norovirus, SILAC, HEK293T,VPg, translation, Calicivirus

curator keyword: Biomedical

submitter keyword: Norovirus, SILAC, HEK293T,VPg, translation, Calicivirus

Contact List

Ian Goodfellow
contact affiliation	Division of Virology, Department of Pathology, University of Cambridge, UK
contact email	ig299@cam.ac.uk
lab head
Edward Emmott
contact affiliation	Northeastern University
contact email	e.emmott@northeastern.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD007585
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD007585

PRIDE project URI

Repository Record List

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