PXD007585 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | AP-MS analysis of murine and human norovirus VPg interactomes |
Description | The norovirus VPg protein is covalently linked to the viral genome in place of a 5' cap, and functions as a cap-substitute, capable of interacting with translation initiation factors. Following on from our previous study (Chung et. al. 2014, J. BIol. Chem.) we wished to determine the interactome of human norovirus VPg, and compare that of murine norovirus VPg. We had previously demonstrated that mutation of the penultimate C-terminal phenylalanine residue in murine norovirus VPg greatly reduced initiation factor binding (F123A). Insertion of the equivalent mutation into human norovirus (F137A) also reduced initiation factor binding. Affinity purification of wild-type of mutant human and murine norovirus VPg was accomplished using GFP-tagged VPg transfected into SILAC-labelled human HEK-293T cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:01:26.985.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Edward Emmott |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 6x(13)C labeled residue |
Instrument | LTQ Orbitrap Velos; Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-08-29 02:09:18 | ID requested | |
1 | 2019-09-11 09:10:10 | announced | |
⏵ 2 | 2024-10-22 04:01:27 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.7554/elife.46681; |
Hosmillo M, Lu J, McAllaster MR, Eaglesham JB, Wang X, Emmott E, Domingues P, Chaudhry Y, Fitzmaurice TJ, Tung MK, Panas MD, McInerney G, Locker N, Wilen CB, Goodfellow IG, Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. Elife, 8():(2019) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Norovirus, SILAC, HEK293T,VPg, translation, Calicivirus |
Contact List
Ian Goodfellow |
contact affiliation | Division of Virology, Department of Pathology, University of Cambridge, UK |
contact email | ig299@cam.ac.uk |
lab head | |
Edward Emmott |
contact affiliation | Northeastern University |
contact email | e.emmott@northeastern.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD007585 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007585
- Label: PRIDE project
- Name: AP-MS analysis of murine and human norovirus VPg interactomes