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PXD007585

PXD007585 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAP-MS analysis of murine and human norovirus VPg interactomes
DescriptionThe norovirus VPg protein is covalently linked to the viral genome in place of a 5' cap, and functions as a cap-substitute, capable of interacting with translation initiation factors. Following on from our previous study (Chung et. al. 2014, J. BIol. Chem.) we wished to determine the interactome of human norovirus VPg, and compare that of murine norovirus VPg. We had previously demonstrated that mutation of the penultimate C-terminal phenylalanine residue in murine norovirus VPg greatly reduced initiation factor binding (F123A). Insertion of the equivalent mutation into human norovirus (F137A) also reduced initiation factor binding. Affinity purification of wild-type of mutant human and murine norovirus VPg was accomplished using GFP-tagged VPg transfected into SILAC-labelled human HEK-293T cells.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:01:26.985.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEdward Emmott
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList6x(13)C labeled residue
InstrumentLTQ Orbitrap Velos; Orbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-08-29 02:09:18ID requested
12019-09-11 09:10:10announced
22024-10-22 04:01:27announced2024-10-22: Updated project metadata.
Publication List
10.7554/elife.46681;
Hosmillo M, Lu J, McAllaster MR, Eaglesham JB, Wang X, Emmott E, Domingues P, Chaudhry Y, Fitzmaurice TJ, Tung MK, Panas MD, McInerney G, Locker N, Wilen CB, Goodfellow IG, Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. Elife, 8():(2019) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Norovirus, SILAC, HEK293T,VPg, translation, Calicivirus
Contact List
Ian Goodfellow
contact affiliationDivision of Virology, Department of Pathology, University of Cambridge, UK
contact emailig299@cam.ac.uk
lab head
Edward Emmott
contact affiliationNortheastern University
contact emaile.emmott@northeastern.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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