PXD007584

PXD007584 is an original dataset announced via ProteomeXchange.

Title	AP-MS analysis of IFIT1 and 5 interactions in interferon-stimulated human cells
Description	This project sought to identify interactions of InterFeron Induced Tetratricopeptide repeat (IFIT) proteins 1 ad 5 in human cells. SILAC-labelled human HEK293T cells were transfected with FLAG-tagged human IFIT1 and IFIT5 pulldowns. After transfection, the cells were treated with human interferon 2-alpha and FLAG-immunoprecipitations performed to identify IFIT-binding proteins. Cells were then subject to LS/MS-MS analysis on a Orbitrap Fusion and data analysed in Maxquant v1.5.7.4
HostingRepository	PRIDE
AnnounceDate	2018-03-16
AnnouncementXML	Submission_2018-04-25_06:31:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edward Emmott
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-29 02:08:37	ID requested
1	2018-03-16 00:57:54	announced
⏵ 2	2018-04-25 06:31:59	announced	Updated publication reference for PubMed record(s): 29554348.

Fleith RC, Mears HV, Leong XY, Sanford TJ, Emmott E, Graham SC, Mansur DS, Sweeney TR, IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA. Nucleic Acids Res, 46(10):5269-5285(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, HEK293T, IFIT, Interferon, SILAC

Trevor Sweeney
contact affiliation	Division of Virology, Department of Pathology, University of Cambridge, Cambridge, UK
contact email	ts629@cam.ac.uk
lab head
Edward Emmott
contact affiliation	University of Cambridge
contact email	ee273@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD007584

PRIDE project URI

• PRIDE
  1. PXD007584
     1. Label: PRIDE project
     2. Name: AP-MS analysis of IFIT1 and 5 interactions in interferon-stimulated human cells