PXD007579

PXD007579 is an original dataset announced via ProteomeXchange.

Title	Comparative proteome analysis of a clonal virulent and attenuated Histomonas meleagridis strain.
Description	The current study aimed to detect and identify significant differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which was in vitro co-cultivated with Escherichia coli DH5α. Two-dimensional gel electrophoresis (2-DE) was used for proteome visualization , gel image software for computational detection of significantly up-regulated protein spots and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) for protein identification. The statistical analysis fulfilling two criteria (> or = 3-fold up-regulation and P<0.05) detected 119 differentially expressed protein spots out of which 62 spots were located in gels of the virulent strain and 57 spots in gels of the attenuated strain. The mass spectrometric analysis of 32 spots, up-regulated in gels of the virulent strain, showed that they are of H. meleagridis origin. As opposed to this, the mass spectrometric analysis of 49 protein spots , up-regulated in the gels of the attenuated strain , identified 32 spots as specific to the protozoan. Additionally, the analysis identified a number of E. coli DH5α proteins which were detected as differentially expressed by the computational gel image and statistical analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:02:22.674.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007579
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ivana BILIC
SpeciesList	scientific name: Histomonas meleagridis; NCBI TaxID: 135588;
ModificationList	deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-28 23:19:39	ID requested
1	2017-12-07 04:51:48	announced
⏵ 2	2024-10-22 04:02:24	announced	2024-10-22: Updated project metadata.

10.1016/j.ijpara.2017.08.017;

Monoyios A, Patzl M, Schlosser S, Hess M, Bilic I, Unravelling the differences: comparative proteomic analysis of a clonal virulent and an attenuated Histomonas meleagridis strain. Int J Parasitol, 48(2):145-157(2018) [pubmed]

10.6019/PXD007579;

submitter keyword: 2-DE, proteome analysis.,Histomonas meleagridis, MALDI-TOF/TOF

Ivana Bilic
contact affiliation	Clinic for Poultry and Fish Medicine University of Veterinary Medicine Vienna Austria
contact email	ivana.bilic@vetmeduni.ac.at
lab head
Ivana BILIC
contact affiliation	Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna
contact email	ivana.bilic@vetmeduni.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007579
     1. Label: PRIDE project
     2. Name: Comparative proteome analysis of a clonal virulent and attenuated Histomonas meleagridis strain.