PXD007577

PXD007577 is an original dataset announced via ProteomeXchange.

Title	The Polycomb group protein CBX6 is an essential regulator of embryonic stem cell identity
Description	Polycomb group proteins are transcriptional repressors that control cell identity and development. In mammals, at least five different CBX proteins are believed to associate with the core Polycomb repressive complex 1 (PRC1). CBX6 and CBX7 are the most highly expressed CBX proteins in embryonic stem cells (ESCs), yet little is known about the function of CBX6 in these cells. Here we show that CBX6 is an essential regulator of ESC identity, since ablation of CBX6 function destabilizes the pluripotency network and triggers differentiation. At the molecular level, CBX6 is linked to the canonical PRC1 (cPRC1) complex; however, contrary to expectations, our results indicate that CBX6 also has a non-canonical PRC1 function. Taken together, our findings reveal that CBX6 is an essential component for ESC biology and contributes to the structural and functional complexity of the PRC1 complex.
HostingRepository	PRIDE
AnnounceDate	2017-09-25
AnnouncementXML	Submission_2017-11-08_04:41:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enrique Blanco
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-28 08:13:57	ID requested
1	2017-09-25 00:52:20	announced
⏵ 2	2017-11-08 04:41:47	announced	Updated publication reference for PubMed record(s): 29089522.

Santanach A, Blanco E, Jiang H, Molloy KR, Sans, ó M, LaCava J, Morey L, Di Croce L, The Polycomb group protein CBX6 is an essential regulator of embryonic stem cell identity. Nat Commun, 8(1):1235(2017) [pubmed]

curator keyword: Biological

submitter keyword: Mouse, ESC, Cbx6, pluripotency, affinity capture followed by MS

Luciano Di Croce
contact affiliation	Epigenetic Events in Cancer (L. Di Croce's lab) Gene Regulation, Stem Cells and Cancer Centre for Genomic Regulation (CRG) C/ Dr. Aiguader, 88 08003 Barcelona Spain
contact email	Luciano.DiCroce@crg.eu
lab head
Enrique Blanco
contact affiliation	CRG
contact email	enrique.blanco@crg.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD007577

PRIDE project URI

• PRIDE
  1. PXD007577
     1. Label: PRIDE project
     2. Name: The Polycomb group protein CBX6 is an essential regulator of embryonic stem cell identity