⮝ Full datasets listing

PXD007575

PXD007575 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMultiplexed thiol reactivity profiling for target discovery of electrophilic natural products
DescriptionElectrophilic groups, such as Michael acceptors, expoxides, are common motifs in natural products (NPs). Electrophilic NPs can act through covalent modification of cysteinyl thiols on functional proteins, and exhibit potent cytotoxicity and anti-inflammatory/cancer activities. Here we describe a new chemoproteomic strategy, termed multiplexed thiol reactivity profiling (MTRP), and its use in target discovery of electrophilic NPs. We demonstrate the utility of MTRP by identifying cellular targets of gambogic acid, an electrophilic NP that is currently under evaluation in clinical trials as anticancer agent. Moreover, MTRP enables simultaneous comparison of seven structurally diversified -unsaturated -lactones, which provides insights into the relative proteomic reactivity and target preference of diverse structural scaffolds coupled to a common electrophilic motif and reveals various potential druggable targets with liganded cysteines. We anticipate that this new method for thiol reactivity profiling in a multiplexed manner will find broad application in redox biology and drug discovery.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:02:22.743.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD007575
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJing Yang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; iTRAQ8plex; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-08-28 07:36:52ID requested
12017-10-12 01:50:12announced
22024-10-22 04:02:28announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.chembiol.2017.08.022;
Tian C, Sun R, Liu K, Fu L, Liu X, Zhou W, Yang Y, Yang J, Multiplexed Thiol Reactivity Profiling for Target Discovery of Electrophilic Natural Products. Cell Chem Biol, 24(11):1416-1427.e5(2017) [pubmed]
10.6019/PXD007575;
Keyword List
curator keyword: Biological
submitter keyword: MTRP;natural products;Chemoproteomics
Contact List
Jing Yang
contact affiliationNational Center for Protein Science Beijing
contact emailyangjing54@hotmail.com
lab head
Jing Yang
contact affiliationNational Center for Protein Sciences, Beijing
contact emailyangjing54@hotmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD007575
PRIDE project URI
Repository Record List
[ + ]