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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-10-12
  • AnnouncementXML: Submission_2017-10-12_01:50:11.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD007575
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Jing Yang
  • Title: Multiplexed thiol reactivity profiling for target discovery of electrophilic natural products
  • Description: Electrophilic groups, such as Michael acceptors, expoxides, are common motifs in natural products (NPs). Electrophilic NPs can act through covalent modification of cysteinyl thiols on functional proteins, and exhibit potent cytotoxicity and anti-inflammatory/cancer activities. Here we describe a new chemoproteomic strategy, termed multiplexed thiol reactivity profiling (MTRP), and its use in target discovery of electrophilic NPs. We demonstrate the utility of MTRP by identifying cellular targets of gambogic acid, an electrophilic NP that is currently under evaluation in clinical trials as anticancer agent. Moreover, MTRP enables simultaneous comparison of seven structurally diversified -unsaturated -lactones, which provides insights into the relative proteomic reactivity and target preference of diverse structural scaffolds coupled to a common electrophilic motif and reveals various potential druggable targets with liganded cysteines. We anticipate that this new method for thiol reactivity profiling in a multiplexed manner will find broad application in redox biology and drug discovery.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: Oxidation; iTRAQ8plex; Carbamidomethyl
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-08-28 07:36:52ID requested
12017-10-12 01:50:12announced

Publication List

  1. Tian C, Sun R, Liu K, Fu L, Liu X, Zhou W, Yang Y, Yang J, Multiplexed Thiol Reactivity Profiling for Target Discovery of Electrophilic Natural Products. Cell Chem Biol, 24(11):1416-1427.e5(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: MTRP
  3. natural products
  4. Chemoproteomics

Contact List

    Jing Yang
    • contact affiliation: National Center for Protein Science Beijing
    • contact email: yangjing54@hotmail.com
    • lab head:
    Jing Yang
    • contact affiliation: National Center for Protein Sciences, Beijing
    • contact email: yangjing54@hotmail.com
    • dataset submitter:

Full Dataset Link List

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