PXD007573

PXD007573 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based proteomics suggests Ephb6 as a potential regulator of the ERK pathway in the prefrontal cortex of chronic social defeat stress model miceiTRAQ-based proteomics reveals Ephb6 as a potential regulator of the ERK pathway in the prefrontal cortex of chronic social defeat stress model mice
Description	Purpose: Major depressive disorder (MDD) is a worldwide concern and devastating psychiatric disease. The World Health Organization claims that MDD leads to at least 11.9% of the global burden of disease. However, the underlying pathophysiology mechanisms of MDD remain largely unknown. Experimental design: Herein, we used a proteomic-based strategy to compare the prefrontal cortex (PFC) in chronic social defeat stress (CSDS) model mice with a control group. Based on pooled samples, differential proteins were identified in the PFC proteome using iTRAQ coupled with LC-MS/MS. Results: Ingenuity Pathway Analysis (IPA) was then followed to predict relevant pathways, with the ephrin receptor signaling pathway selected for further research. Additionally, as the selected key proteins of the ephrin receptor signaling pathway, ephrin type-B receptor 6 (EphB6) and the ERK pathway were validated by western blotting. Conclusion and clinical relevant: Altogether, increased understanding of the ephrin receptor signaling pathway in MDD is provided, which implicates further investigation of PFC dysfunction induced by CSDS treatment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:02:32.854.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guo Hua
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-28 07:30:12	ID requested
1	2018-07-05 06:57:44	announced
⏵ 2	2024-10-22 04:02:40	announced	2024-10-22: Updated project metadata.

Guo H, Huang ZL, Wang W, Zhang SX, Li J, Cheng K, Xu K, He Y, Gui SW, Li PF, Wang HY, Dong ZF, Xie P, iTRAQ-Based Proteomics Suggests Ephb6 as a Potential Regulator of the ERK Pathway in the Prefrontal Cortex of Chronic Social Defeat Stress Model Mice. Proteomics Clin Appl, 11(11-12):(2017) [pubmed]

10.1002/prca.201700115;

curator keyword: Biomedical

submitter keyword: major depressive disorder, prefrontal cortex,chronic social defeat stress, EphB6, iTRAQ

Xie Peng
contact affiliation	Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
contact email	xiepeng@cqmu.edu.cn
lab head
Guo Hua
contact affiliation	Chongqing Medical university
contact email	344679464@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007573
     1. Label: PRIDE project
     2. Name: iTRAQ-based proteomics suggests Ephb6 as a potential regulator of the ERK pathway in the prefrontal cortex of chronic social defeat stress model miceiTRAQ-based proteomics reveals Ephb6 as a potential regulator of the ERK pathway in the prefrontal cortex of chronic social defeat stress model mice