PXD007569

PXD007569 is an original dataset announced via ProteomeXchange.

Title	Human islet of Langerhans grown on nanosubstrates LC-MSMS
Description	Intensive efforts are focused on identifying regulators of human pancreatic islet cell growth and maturation to accelerate development of therapies for diabetes. In the present study we evaluate the contribution of the physical properties of the extracellular matrix in supporting long term culture of human islets in vitro. We used metal oxide layers with tailored nanoscale roughness to fabricate scaffolds. Their suitability to support long term culture of human islets was investigated through assessment of β-cell survival, differentiation and function by using a proteomic approach to evaluate the molecular mechanisms involved. Proteomic analysis confirmed that ns-ZrOx promoted the activation of a transcriptional and translational program leading to activation of pro-survival and pro-differentiation signalling pathways. The process was driven by a mechanotransductive pathway driven by nanostructured topology, via remodelling of the actin cytoskeleton nuclear architecture and increase of oxygen sensor PDH2 Our data suggest that β-cells relay on nanotopography features to regulate their differentiation and survival. Tailored nanostructured substrates may provide a unique strategy to identify novel hints for tissue engineering and molecular /pharmacological targets of intervention to treat diabetes mellitus.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:02:08.633.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	gabriella tedeschi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; phosphorylated residue; iodoacetic acid derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-28 03:53:21	ID requested
1	2018-07-11 03:54:04	announced
⏵ 2	2024-10-22 04:02:12	announced	2024-10-22: Updated project metadata.

10.1038/s41598-018-28019-3;

Galli A, Maffioli E, Sogne E, Moretti S, Di Cairano ES, Negri A, Nonnis S, Norata GD, Bonacina F, Borghi F, Podest, à A, Bertuzzi F, Milani P, Lenardi C, Tedeschi G, Perego C, Cluster-assembled zirconia substrates promote long-term differentiation and functioning of human islets of Langerhans. Sci Rep, 8(1):9979(2018) [pubmed]

curator keyword: Biological

submitter keyword: Human, LC-MSMS, islet of Langerhans, nanosubstrates

Gabriella Tedeschi
contact affiliation	Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milano, Italy
contact email	gabriella.tedeschi@unimi.it
lab head
gabriella tedeschi
contact affiliation	University of Milano
contact email	gabriella.tedeschi@unimi.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD007569

PRIDE project URI

• PRIDE
  1. PXD007569
     1. Label: PRIDE project
     2. Name: Human islet of Langerhans grown on nanosubstrates LC-MSMS