PXD007567

PXD007567 is an original dataset announced via ProteomeXchange.

Title	Combined proteomics/genomics approach reveals proteomic changes of mature virions as a novel poxvirus adaption mechanism
Description	DNA viruses, like poxviruses, possess a highly stable genome, suggesting adaption of virus particles to specific cell types is not restricted to genomic changes. Cowpox viruses (CPXV) are zoonotic poxviruses with an extraordinary broad host range, demonstrating their adaptive potential in vivo. To elucidate novel adaption mechanisms of poxviruses, we isolated CPXV particles from a rat and passaged them five times in a human (HEp-2) and a rat (Rat-2) cell line. Subsequently, we purified mature virions and compared the proteome of the non-passaged virions and each passage.
HostingRepository	PRIDE
AnnounceDate	2017-11-14
AnnouncementXML	Submission_2017-11-14_01:02:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marica Grossegesse
SpeciesList	scientific name: Cowpox virus (CPV); NCBI TaxID: 10243;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitap Discovery

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-28 02:05:18	ID requested
⏵ 1	2017-11-14 01:02:11	announced

Grossegesse M, Doellinger J, Tyshaieva A, Schaade L, Nitsche A, Combined Proteomics/Genomics Approach Reveals Proteomic Changes of Mature Virions as a Novel Poxvirus Adaptation Mechanism. Viruses, 9(11):(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Poxvirus, Cowpox virus, adaption, LC-MS/MS

Andreas Nitsche
contact affiliation	Robert Koch Institute, Centre for Biological Threats and Special Pathogens (ZBS1), Berlin, Germany
contact email	NitscheA@rki.de
lab head
Marica Grossegesse
contact affiliation	Robert Koch Institute
contact email	GrossegesseM@rki.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007567

PRIDE project URI

• PRIDE
  1. PXD007567
     1. Label: PRIDE project
     2. Name: Combined proteomics/genomics approach reveals proteomic changes of mature virions as a novel poxvirus adaption mechanism