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PXD007556

PXD007556 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtease specificity profiling in a pipette tip using “charge-synchronized” proteome-derived peptide libraries
DescriptionAbout 2% of the genome of human and other organisms codes for proteases. An important step toward deciphering the biological function of a protease and designing inhibitors is the profiling of protease specificity. In this work we present a novel, label-free, proteomics-based protease specificity profiling method that only requires simple sample preparation steps. It uses proteome-derived peptide libraries and enriches the cleaved sequences using strong cation exchange chromatography (SCX) material in a pipette tip. As a demonstration of the method’s versatility, we successfully determined the specificity of GluC, caspase-3, chymotrypsin and cathepsin G from several hundreds to almost 2000 cleavage events per proteases. Interestingly, we also found and confirmed a novel intrinsic preference of cathepsin G for Asn at the P1 subsite, explaining the reported cleavage position on the P. aeruginosa aeruginosa flagellin by human cathepsin G . Overall, this method is straightforward and requires so far the lowest investment in material and equipment for protease specificity profiling. Therefore, we think it will be applicable in any biochemistry laboratory and promote an increased understanding of protease specificity.
HostingRepositoryPRIDE
AnnounceDate2018-04-26
AnnouncementXMLSubmission_2018-04-26_05:56:53.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMinh Thi Nhat Nguyen
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListacetylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-08-28 00:57:20ID requested
12018-04-26 05:56:54announced
Publication List
Nguyen MTN, Shema G, Zahedi RP, Verhelst SHL, Protease Specificity Profiling in a Pipet Tip Using "Charge-Synchronized" Proteome-Derived Peptide Libraries. J Proteome Res, 17(5):1923-1933(2018) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: ChaFRAtip, protease specificity, label-free, charged-synchronized peptide library
Contact List
Steven L. Verhelst
contact affiliationProf. Dr. Steven Verhelst Laboratory of Chemical Biology Department of Cellular and Molecular Medicine KU Leuven - University of Leuven Herestraat 49, box 802 3000 Leuven Belgium
contact emailstevenverhelst@kuleuven.de
lab head
Minh Thi Nhat Nguyen
contact affiliationLeibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact emailminh.nguyen@isas.de
dataset submitter
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