PXD007546

PXD007546 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS analysis of Mannose 6-phosphate containing proteins
Description	Mucolipidosis III gamma (MLIII) is clinically characterized by onset of first symptoms at an average of 5 years such as stiffness of hands and shoulders, claw hand deformities, scoliosis and progressive destruction of hip joints. The disease is caused by mutations in GNPTG encoding the gamma-subunit of the GlcNAc-1-phosphotransferase complex. This enzyme is responsible for the generation of mannose 6-phosphate (M6P) targeting signals on 70 soluble lysosomal enzymes that are required for their efficient receptor-mediated transport to lysosomes. In fibroblasts from Gnptg-KO mice the GlcNAc-1-phosphotransferase activity is reduced leading to low amounts of M6P-containing proteins. M6P affinity chromatography-assisted and mass spectrometry-based secretome analysis of Gnptg-KO fibroblasts revealed a distinct set of lysosomal enzymes modified with M6P residues.
HostingRepository	PRIDE
AnnounceDate	2018-06-06
AnnouncementXML	Submission_2018-06-06_03:08:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shiva Ahmadi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-24 03:40:46	ID requested
⏵ 1	2018-06-06 03:08:04	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: : mouse embryonic fibroblasts, secreted proteins, mannose 6-phosphate affinity purification

Dominic Winter
contact affiliation	Principal Investigator (PI)
contact email	dwin@uni-bonn.de
lab head
Shiva Ahmadi
contact affiliation	Researcher
contact email	sheeva.ahmadi@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD007546

PRIDE project URI

• PRIDE
  1. PXD007546
     1. Label: PRIDE project
     2. Name: LC-MS/MS analysis of Mannose 6-phosphate containing proteins