PXD007533

PXD007533 is an original dataset announced via ProteomeXchange.

Title	Vitamin C inhibits bacterial biofilms by affecting the synthesis of the extracellular polymeric matrix
Description	Extracellular polymeric substances (EPS) produced by bacteria form a matrix for the complex three-dimensional architecture of biofilms. The EPS matrix of a biofilm is primarily composed of polysaccharides, proteins and extracellular DNA (eDNA). In addition to supporting the community structure, the EPS matrix protects bacterial biofilms from the environment. Specifically, the EPS matrix reduces the penetration of antimicrobial agents into the biofilm, thereby reducing their efficiency. Strategies for disrupting the formation of the EPS matrix can therefore lead to a more efficient use of antimicrobial agents. Here, we examine the biofilm-disrupting activity of vitamin C (sodium ascorbate). Quantitative proteomics analysis of vitamin C treated biofilms show that exposure to vitamin C inhibits quorum sensing and other regulatory mechanisms underpinning biofilm development. This results in inhibition of the EPS biosynthesis, and depletion of the polysaccharide component of the matrix. Once the EPS content is reduced beyond a critical point, bacterial cells get fully exposed to the medium, thereby experiencing a planktonic-like state. At this stage, the cells are more susceptible to killing, either by vitamin C-induced oxidative stress as reported here, or by other antibacterial compounds or treatments.
HostingRepository	PRIDE
AnnounceDate	2017-12-19
AnnouncementXML	Submission_2017-12-19_02:51:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vaishnavi Ravikumar
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-22 04:00:33	ID requested
⏵ 1	2017-12-19 02:51:03	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: bacteria, label-free quantification, mass spectrometry

Ivan Mijakovic
contact affiliation	Professor Director of Chalmers Area of Advance Life Science Engineering Systems and Synthetic Biology Chalmers University of Technology Kemivägen 10 SE-412 96 Göteborg SWEDEN
contact email	ivan.mijakovic@chalmers.se
lab head
Vaishnavi Ravikumar
contact affiliation	Technical University of Denmark
contact email	vairav@biosustain.dtu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD007533

PRIDE project URI

• PRIDE
  1. PXD007533
     1. Label: PRIDE project
     2. Name: Vitamin C inhibits bacterial biofilms by affecting the synthesis of the extracellular polymeric matrix