PXD007532 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS analysis of human thyroid cystic fluid and thyroid cancer cell secreted proteins |
Description | Thyroid ultrasound and ultrasound-guided fine-needle aspiration (USG/FNA) biopsy are currently used for diagnosing papillary thyroid carcinoma (PTC), but their detection limit could be improved by combining other biomarkers. To discover novel PTC biomarkers, we herein applied a GeLC-MS/MS strategy to analyze the proteome profiles of serum-abundant-protein-depleted FNA cystic fluid from benign and PTC patients, as well as two PTC cell line secretomes. From them, we identified 346, 488, and 2105 proteins, respectively. Comparative analysis revealed that 191 proteins were detected in the PTC but not the benign cystic fluid samples, and thus may represent potential PTC biomarkers. Among these proteins, 101 were detected in the PTC cell line secretomes, and seven of them (NPC2, CTSC, AGRN, GPNMB, DPP4, ERAP2, and SH3BGRL3) were reported in public PTC transcriptome datasets as having 4681 elevated mRNA expression in PTC. Immunoblot analysis confirmed the elevated expression levels of five proteins (NPC2, CTSC, GPNMB, DPP4, and ERAP2) in PTC versus benign cystic fluids. Immunohistochemical studies from near 100 pairs of PTC tissue and their adjacent non-tumor counterparts further showed that AGRN (n = 98), CTSC (n = 99), ERAP2 (n = 98) and GPNMB (n = 100) were significantly (p<0.05) overexpressed in PTC and higher expression levels of AGRN and CTSC were also significantly associated with metastasis and poor prognosis of PTC patients. Collectively, our results indicate that an integrated analysis of FNA cystic fluid proteome, cancer cell secretome and tissue transcriptome datasets represents a useful strategy for efficiently discovering novel PTC biomarker candidates. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_03:59:38.849.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chia-Chun Wu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-08-22 03:58:27 | ID requested | |
1 | 2018-03-21 02:00:50 | announced | |
⏵ 2 | 2024-10-22 03:59:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.18632/oncotarget.23951; |
Wu CC, Lin JD, Chen JT, Chang CM, Weng HF, Hsueh C, Chien HP, Yu JS, Integrated analysis of fine-needle-aspiration cystic fluid proteome, cancer cell secretome, and public transcriptome datasets for papillary thyroid cancer biomarker discovery. Oncotarget, 9(15):12079-12100(2018) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Human, Thyroid cystic fluid, Thyroid cancer cell, LC-MS/MS |
Contact List
Jau Song Yu |
contact affiliation | Graduate Institute of Biomedical Sciences, department of Cell and Molecular Biology, College of Medicine, and Molecular Medicine Research Center, Chang Gung University, Taoyuan 33302, Taiwan. Liver Research Center, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan 33302, Taiwan. |
contact email | yusong@mail.cgu.edu.tw |
lab head | |
Chia-Chun Wu |
contact affiliation | Molecular Medicine Research Center, Chang Gung University |
contact email | wuchiachun9@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD007532 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007532
- Label: PRIDE project
- Name: LC-MS/MS analysis of human thyroid cystic fluid and thyroid cancer cell secreted proteins