PXD007525

PXD007525 is an original dataset announced via ProteomeXchange.

Title	Characterisation of nuclear and sub-nuclear proteomes of Saccharomyces cerevisiae
Description	This study concerns characterisation of the nuclear, chromatin and nuclear non-chromatin proteomes of the yeast Saccharomyces cerevisiae. We performed nuclear and chromatin enrichments from exponentially growing cells using differential centrifugation and analysed the nuclear and chromatin pellets, along with their accompanying supernatants, by LC-MS/MS on two different platforms. We inferred relative quantitation of proteins using emPAI and compared the relative amounts of proteins in each of our samples which enabled us to infer probable residents of the nuclear and chromatin proteomes.
HostingRepository	PRIDE
AnnounceDate	2019-11-07
AnnouncementXML	Submission_2019-11-06_23:37:08.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007525
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Daniel Nightingale
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-22 02:37:39	ID requested
⏵ 1	2019-11-06 23:42:52	announced

Dikicioglu D, Nightingale DJH, Wood V, Lilley KS, Oliver SG, Transcriptional regulation of the genes involved in protein metabolism and processing in Saccharomyces cerevisiae. FEMS Yeast Res, 19(2):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Saccharomyces cerevisiae, nucleus, chromatin, organelle, LC-MS/MS, shotgun proteomics

Kathryn Susan Lilley
contact affiliation	Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, United Kingdom
contact email	k.s.lilley@bioc.cam.ac.uk
lab head
Daniel Nightingale
contact affiliation	University of Cambridge
contact email	djhn2@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD007525

PRIDE project URI

• PRIDE
  1. PXD007525
     1. Label: PRIDE project
     2. Name: Characterisation of nuclear and sub-nuclear proteomes of Saccharomyces cerevisiae