PXD007515

PXD007515 is an original dataset announced via ProteomeXchange.

Title	Discovery of a testis-specific complex TEX101-DPEP3 and selection of its disrupting antibodies
Description	TEX101 is a testis-specific protein expressed exclusively in male germ cells and is a validated biomarker of male infertility. Studies in mice suggest that TEX101 is a cell-surface chaperone which regulates, through protein-protein interactions, the maturation of proteins involved in spermatozoa transit and oocyte binding. Male TEX101-null mice are sterile. Here, we identified by co-immunoprecipitation-mass spectrometry the interactome of human TEX101 in testicular tissues and spermatozoa. The testis-specific cell-surface dipeptidase 3 (DPEP3) emerged as the top hit. We further validated the TEX101-DPEP3 complex by using hybrid immunoassays. Combinations of antibodies recognizing different epitopes of TEX101 and DPEP3 facilitated development of a simple immunoassay to screen for disruptors of TEX101-DPEP3 complex. As a proof-of-a-concept, we demonstrated that anti-TEX101 clone 34ED604 (antibody T4) disrupted the native TEX101-DPEP3 complex. Disrupting antibodies may be used to study the human TEX101-DPEP3 complex, and to develop modulators for male fertility.
HostingRepository	PRIDE
AnnounceDate	2018-08-16
AnnouncementXML	Submission_2018-08-16_09:37:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Schiza
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-18 01:00:31	ID requested
⏵ 1	2018-08-16 09:37:35	announced

Schiza C, Korbakis D, Panteleli E, Jarvi K, Drabovich AP, Diamandis EP, Discovery of a Human Testis-specific Protein Complex TEX101-DPEP3 and Selection of Its Disrupting Antibodies. Mol Cell Proteomics, 17(12):2480-2495(2018) [pubmed]

submitter keyword: TEX101

testis-expressed sequence 101 protein

DPEP3

dipeptidase 3

affinity purification-mass spectrometry

interactome

protein-protein interactions

testis-specific proteins

spermatozoa

disrupting antibodies

Eleftherios P. Diamandis
contact affiliation	Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada;Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Canada;Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Canada;Department of Clinical Biochemistry, University Health Network, Toronto, Canada
contact email	eleftherios.diamandis@sinaihealthsystem.ca
lab head
Christina Schiza
contact affiliation	University of Toronto
contact email	sxristina2011@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007515
     1. Label: PRIDE project
     2. Name: Discovery of a testis-specific complex TEX101-DPEP3 and selection of its disrupting antibodies