PXD007511

PXD007511 is an original dataset announced via ProteomeXchange.

Title	The interactomes of RUNX1 and RUNX3 from Mus musculus T cells
Description	Cellular binary fate decisions require the progeny to silence genes associated with the alternative fate. The major subsets of alpha:beta T cells have been extensively studied as a model system for fate decisions. While the transcription factor RUNX3 is required for the initiation of Cd4 silencing in CD8 T cell progenitors, it is not required to maintain the silencing of Cd4 and other helper T lineage genes. The other runt domain containing protein, RUNX1, silences Cd4 in an earlier T cell progenitor, but this silencing is reversed whereas the gene silencing after RUNX3 expression is not reverse. Therefore, we hypothesized that RUNX3 and not RUNX1 recruits other factors that maintains the silencing of helper T lineage genes in CD8 T cells. To this end, we performed a proteomics screen of RUNX1 and RUNX3 to determine candidate silencing factors.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_00:42:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Verbaro
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-17 23:59:58	ID requested
⏵ 1	2019-11-12 00:42:58	announced

Verbaro DJ, Sakurai N, Kim B, Shinkai Y, Egawa T, Cutting Edge: The Histone Methyltransferase G9a Is Required for Silencing of Helper T Lineage-Associated Genes in Proliferating CD8 T Cells. J Immunol, 200(12):3891-3896(2018) [pubmed]

curator keyword: Biological

submitter keyword: Mus musculus RUNX1 RUNX3 Immunoprecipitation T cells

Takeshi Egawa
contact affiliation	Pathology and Immunology, Washington University in St. Louis, St. Louis, MO
contact email	egawat@wustl.edu
lab head
Daniel Verbaro
contact affiliation	Washington University in St. Louis
contact email	verbaro.d@wustl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD007511

PRIDE project URI

• PRIDE
  1. PXD007511
     1. Label: PRIDE project
     2. Name: The interactomes of RUNX1 and RUNX3 from Mus musculus T cells