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PXD007284

PXD007284 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSubstrate identification of ADAMTS16
DescriptionSecreted and cell-surface proteases are major mediators of extracellular matrix remodelling, but their mechanisms and regulatory impact are poorly understood. We developed a mass spectrometry approach using cell free ECM produced by mouse Balb/c 3T3 cells in vitro to identify fibronectin as a novel substrate of the secreted metalloprotease ADAMTS16. ADAMTS16 cleaves fibronectin between its (I)5 and (I)6 modules, releasing the N-terminal 30kDa heparin-binding domain essential for fibronectin assembly.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:14:30.136.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRahel Schnellmann
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-08-16 23:54:57ID requested
12018-04-23 04:10:31announced
22024-10-22 04:14:32announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.ra118.000676;
Schnellmann R, Sack R, Hess D, Annis DS, Mosher DF, Apte SS, Chiquet-Ehrismann R, A Selective Extracellular Matrix Proteomics Approach Identifies Fibronectin Proteolysis by A Disintegrin-like and Metalloprotease Domain with Thrombospondin Type 1 Motifs (ADAMTS16) and Its Impact on Spheroid Morphogenesis. Mol Cell Proteomics, 17(7):1410-1425(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: ADAMTS16, Fibronectin, LC-MS/MS
Contact List
Suneel S. Apte
contact affiliationCleveland Clinic Lerner Research Institute
contact emailaptes@ccf.org
lab head
Rahel Schnellmann
contact affiliationFriedrich Miescher Institue
contact emailrahel.schnellmann@fmi.ch
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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