PXD007278

PXD007278 is an original dataset announced via ProteomeXchange.

Title	Comprehensive redox profiling of the thiol proteome of Clostridium difficile
Description	The strictly anaerobic bacterium C. difficile has become one of the most problematic hospital acquired pathogens and a major burden for health care systems. Although antibiotics work effectively in most C. difficile infections (CDIs), their detrimental effect on the intestinal microbiome paves the way for recurrent episodes of CDI. To develop alternative, non-antibiotics-based treatment strategies, deeper knowledge on the physiology of C. difficile, stress adaptation mechanisms and regulation of virulence factors is mandatory. Focus of this work was to tackle the thiol proteome of C. difficile and its stress-induced alterations, since recent research reported the amino acid cysteine to play a central role in the metabolism of the pathogen. We developed a novel cysteine labeling approach to determine the redox state of protein thiols on a global scale. Applicability of the technique was demonstrated by inducing disulfide stress using the chemical diamide. The method can be transferred to any kind of redox challenge and was applied in this work to assess the effect of bile acids on the thiol proteome of C. difficile. We present redox-quantification of more than 1,500 thiol peptides and discuss the general difficulty of redox analyses of peptides possessing more than a single cysteine residue. The presented method will be especially useful when not only redox status shall be determined, but information on protein quantity is needed as well. Not least, our comprehensive dataset reveals protein cysteine sites particularly susceptible to oxidation and builds a groundwork for redox proteomics studies in C. difficile.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:14:17.658.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susanne Sievers
SpeciesList	scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: 272563;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-16 00:47:15	ID requested
1	2018-03-05 00:22:33	announced
⏵ 2	2024-10-22 04:14:18	announced	2024-10-22: Updated project metadata.

10.1074/mcp.tir118.000671;

Sievers S, Dittmann S, Jordt T, Otto A, Hochgr, ä, fe F, Riedel K, . Mol Cell Proteomics, 17(5):1035-1046(2018) [pubmed]

curator keyword: Biological

submitter keyword: thiol redox proteomics, Clostridium difficile

Susanne Sievers
contact affiliation	Institute of Microbiology, Department of Microbial Physiology & Molecular Biology, University of Greifswald, Felix-Hausdorff Str. 8, 17489 Greifswald, phone: ++49 3834 420 5913
contact email	susanne.sievers@uni-greifswald.de
lab head
Susanne Sievers
contact affiliation	University of Greifswald
contact email	susanne.sievers@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD007278

PRIDE project URI

• PRIDE
  1. PXD007278
     1. Label: PRIDE project
     2. Name: Comprehensive redox profiling of the thiol proteome of Clostridium difficile