PXD007273

PXD007273 is an original dataset announced via ProteomeXchange.

Title	Single-shot top-down proteomics with capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for identification of nearly 600 Escherichia coli proteoforms
Description	Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) has been recognized as an invaluable platform for top-down proteomics. However, the scale of top-down proteomics from CZE-MS/MS is still limited due to the low loading capacity and narrow separation window of CZE. In this work, for the first time we systematically evaluated dynamic pH junction method for focusing of intact proteins during CZE-MS. The optimized dynamic pH junction based CZE-MS/MS system approached 1-µL loading capacity, 90-min separation window and high peak capacity (~280) for separation of Escherichia coli proteome. The results represent the largest loading capacity and the highest peak capacity of CZE for top-down characterization of complex proteomes. About 2,800 proteoform-spectrum matches, nearly 600 proteoforms, and 200 proteins were identified from an Escherichia coli lysate by single-shot CZE-MS/MS with spectrum-level false discovery rate (FDR) less than 1%. The number of proteoforms is over three times higher than that from previous single-shot CZE-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:14:16.295.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liangliang Sun
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-15 01:43:27	ID requested
1	2017-10-30 03:13:57	announced
⏵ 2	2024-10-22 04:14:17	announced	2024-10-22: Updated project metadata.

10.1021/acs.analchem.7b02532;

Lubeckyj RA, McCool EN, Shen X, Kou Q, Liu X, Sun L, Single-Shot Top-Down Proteomics with Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry for Identification of Nearly 600 Escherichia coli Proteoforms. Anal Chem, 89(22):12059-12067(2017) [pubmed]

curator keyword: Technical, Biological

submitter keyword: top-down proteomics,CZE-MS/MS, E.coli

Liangliang Sun
contact affiliation	Michigan State University
contact email	lsun@chemistry.msu.edu
lab head
Liangliang Sun
contact affiliation	Michigan State University
contact email	lsun@chemistry.msu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007273
     1. Label: PRIDE project
     2. Name: Single-shot top-down proteomics with capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for identification of nearly 600 Escherichia coli proteoforms