PXD007256

PXD007256 is an original dataset announced via ProteomeXchange.

Title	Calcium/calmodulin dependent protein kinase kinase 2 (CAMKK2), an alternative to curcumin in head and neck squamous cell carcinoma.
Description	Curcumin has been shown to exhibit anti-neoplastic effects. However, due to its poor bioavailability, use of curcumin as an anti-cancer drug is limited. It is thus necessary to identify molecules that could be targeted using anti-cancer agents as alternatives to curcumin. For this, it is important to understand the underlying signaling pathways mediating the anti-neoplastic effects of curcumin. We carried out phosphoproteomic analysis (TiO2-based enrichment of Ser/Thr kinases) of head and neck cancer cell line, CAL27 treated with curcumin to identify curcumin mediated signaling pathways. This resulted in the identification of 5921 phosphopeptides (phosphosite probability≥75%) corresponding 1,878 proteins. Of these, 275 and 335 phosphopeptides corresponding to 183 and 242 proteins (≥2.0-fold) were found to be hyper- and hypo-phosphorylated respectively, in response to curcumin treatment
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:23.021.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aditi Chatterjee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-12 02:20:35	ID requested
1	2022-03-03 01:30:22	announced
⏵ 2	2023-11-14 08:49:23	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: bioavailability, head and neck cancer, mass spectrometry, signaling pathways,Kinases, phosphoproteomics, quantitative proteomics

Aditi Chatterjee
contact affiliation	Institute of Bioinformatics, ITPB, Bangalore, India
contact email	aditixchatterjee@gmail.com
lab head
Aditi Chatterjee
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India
contact email	aditixchatterjee@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007256
     1. Label: PRIDE project
     2. Name: Calcium/calmodulin dependent protein kinase kinase 2 (CAMKK2), an alternative to curcumin in head and neck squamous cell carcinoma.