PXD007255

PXD007255 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Fasciola hepatica somatic proteome and secretome
Description	Background The parasite Fasciola hepatica infects a broad range of mammals with impunity. Following ingestion of parasites (metacercariae) by the host, newly excysted juveniles (NEJ) emerge from their cysts, rapidly penetrate the duodenal wall and migrate to the liver. Successful infection takes just a few hours and involves negotiating hurdles presented by host macromolecules, tissues and micro-environments, as well as the immune system. Results Transcriptome and proteome analysis of F. hepatica metacercariae and NEJ revealed the rapidity and multitude of metabolic and developmental alterations this parasite undergoes to accomplish infection. We show that metacercariae are metabolically active, contrary to the view that this stage is dormant. Following ingestion, the NEJ expend vital energy stores and rapidly adjust their metabolic pathways to cope with their new increasingly anaerobic environment. Temperature increases induce neoblast proliferation and the remarkable up-regulation of genes associated with growth and development. Cysteine proteases synthesised by gastrodermal cells are secreted to facilitate invasion and tegumental transporters are varied to deal with osmotic/salinity changes. Major proteins of the total NEJ secretome include proteases, proteases inhibitors, anti-oxidants as well as an array of immunomodulators that likely disarm host innate immune effector cells. Conclusions The challenges of infection by F. hepatica parasites are met by rapid metabolic and physiological adjustments that expedite tissue invasion and immune evasion that facilitate growth, development and maturation. Our molecular analysis of the critical process of host invasion has identified key targets for future drug and vaccine strategies directed at preventing parasite infection.
HostingRepository	PRIDE
AnnounceDate	2018-03-26
AnnouncementXML	Submission_2018-03-26_07:41:26.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007255
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Krystyna Cwiklinski
SpeciesList	scientific name: Fasciola hepatica (Liver fluke); NCBI TaxID: 6192;
ModificationList	Glu->pyro-Glu; Deamidated; Oxidation; Carbamidomethyl; Gln->pyro-Glu
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-12 02:15:53	ID requested
⏵ 1	2018-03-26 07:41:27	announced

Cwiklinski K, Jewhurst H, McVeigh P, Barbour T, Maule AG, Tort J, O'Neill SM, Robinson MW, Donnelly S, Dalton JP, Requires Rapid Regulation of Metabolic, Virulence, and Invasive Factors to Adjust to Its Mammalian Host. Mol Cell Proteomics, 17(4):792-809(2018) [pubmed]

curator keyword: Biological

submitter keyword: Fasciola hepatica, metacercariae, NEJ, somatic proteome, secretome, ES proteins

John P Dalton
contact affiliation	Professor in Infectious Diseases School of Biological Sciences Medical Biology Centre (MBC) Queen's University Belfast Belfast UK
contact email	j.dalton@qub.ac.uk
lab head
Krystyna Cwiklinski
contact affiliation	Queen's University Belfast
contact email	k.cwiklinski@qub.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007255
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Fasciola hepatica somatic proteome and secretome