PXD007231 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Stable isotope dynamic labelling of secretomes C4PR_LIV |
Description | We improve on currently-available resources by describing a mass spectrometry (MS)-based strategy using stable isotope dynamic labelling of secretomes (SIDLS) that discriminates between authentic secretory proteins and intracellular proteins within the secretome of cultured cells. By monitoring the rate of incorporation of labelled amino acids into newly synthesised proteins as they appear in the media, we can differentiate those proteins that have been destined for secretion, and exhibit rapid labelling, from those with low rates of labelling or low turnover relative to the growth rate of the cells which is a feature of intracellular proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:12:25.495.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dean Hammond |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-08-09 05:59:19 | ID requested | |
1 | 2018-08-20 02:58:26 | announced | |
2 | 2019-10-08 02:08:18 | announced | 2019-10-08: Updated publication reference for PubMed record(s): 29915148. |
3 | 2019-11-27 06:27:42 | announced | 2019-11-27: Updated project metadata. |
⏵ 4 | 2024-10-22 04:12:31 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.TIR117.000516; |
Hammond DE, Kumar JD, Raymond L, Simpson DM, Beynon RJ, Dockray GJ, Varro A, Stable Isotope Dynamic Labeling of Secretomes (SIDLS) Identifies Authentic Secretory Proteins Released by Cancer and Stromal Cells. Mol Cell Proteomics, 17(9):1837-1849(2018) [pubmed] |
Keyword List
curator keyword: Technical, Biomedical |
submitter keyword: Human Cancer Cells Secretome Dynamic Labelling |
Contact List
Dean Hammond |
contact affiliation | Institute of Translational Medicine University of Liverpool Crown Street Liverpool L69 3BX |
contact email | d.e.hammond@liverpool.ac.uk |
lab head | |
Dean Hammond |
contact affiliation | University of Liverpool |
contact email | d.e.hammond@liv.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD007231 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007231
- Label: PRIDE project
- Name: Stable isotope dynamic labelling of secretomes C4PR_LIV