PXD007230

PXD007230 is an original dataset announced via ProteomeXchange.

Title	Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling
Description	Following endocytosis into the endosomal network, integral membrane proteins undergo sorting for lysosomal degradation or are retrieved and recycled back to the cell surface. Here we describe the discovery of an ancient and conserved multiprotein complex which orchestrates cargo retrieval and recycling and importantly, is biochemically and functionally distinct to the established retromer pathway. Composed of a heterotrimer of DSCR3, C16orf62 and VPS29, and bearing striking similarity with retromer, we have called this complex ‘retriever’. We establish that retriever associates with the cargo adaptor sorting nexin 17 (SNX17) and couples to CCC (CCDC93, CCDC22, COMMD) and WASH complexes to prevent lysosomal degradation and promote cell surface recycling of α5β1-integrin. Through quantitative proteomic analysis we identify over 120 cell surface proteins, including numerous integrins, signalling receptors and solute transporters, which require SNX17-retriever to maintain their surface levels. Our identification of retriever establishes a major endosomal retrieval and recycling pathway.
HostingRepository	PRIDE
AnnounceDate	2018-01-15
AnnouncementXML	Submission_2018-01-15_00:06:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kerrie McNally
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-09 05:57:27	ID requested
⏵ 1	2018-01-15 00:06:18	announced

McNally KE, Faulkner R, Steinberg F, Gallon M, Ghai R, Pim D, Langton P, Pearson N, Danson CM, N, ä, gele H, Morris LL, Singla A, Overlee BL, Heesom KJ, Sessions R, Banks L, Collins BM, Berger I, Billadeau DD, Burstein E, Cullen PJ, Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling. Nat Cell Biol, 19(10):1214-1225(2017) [pubmed]

curator keyword: Biological

submitter keyword: retriever, retromer, SNX17

Peter J Cullen
contact affiliation	University of Bristol, School of Biochemistry
contact email	Pete.Cullen@bristol.ac.uk
lab head
Kerrie McNally
contact affiliation	University of Bristol
contact email	km13610@bristol.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD007230

PRIDE project URI

• PRIDE
  1. PXD007230
     1. Label: PRIDE project
     2. Name: Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling