PXD007228

PXD007228 is an original dataset announced via ProteomeXchange.

Title	QUANTITATIVE PHOSPHOPROTEOMIC PROFILING OF PRIMARY RETINOBLASTOMA TUMORS
Description	Retinoblastoma is a malignant tumor of the retina which most often occurs in children below 5 years of age with an incident rate of about 1 in 15,000 to 18,000 live births. Retinoblastoma is the first ever cancer that was reported to have a genetic basis. It occurs widely due to inactivating mutations in RB1 gene. Gene expression studies, copy number variation analysis, epigenetic profiling including miRNA and methylation of retinoblastoma has been carried to understand the disease mechanism and key players in the disease. Our group has earlier performed differential proteomics of retinoblastoma to identify proteins of therapeutic importance. However, there are no studies to understand the signalling mechanisms associated with retinoblastoma. Hence, global phosphoproteomics of retinoblastoma was carried out to identify signalling events associated with this cancer. Our study identified stress response proteins to be hyper phosphorylated which included H2AFX and sirtuin 1. In particular, Ser140 of H2AFX also known as gamma-H2AX was found to be hyperphosphorylated in retinoblastoma that indicated activation of DNA damage response pathways. We also observed activation of anti-apoptotic proteins in retinoblastoma compared to control. These observations showed activation of survival pathways and signalling networks activated in tumors.
HostingRepository	PRIDE
AnnounceDate	2019-11-11
AnnouncementXML	Submission_2019-11-11_04:31:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sailaja Elchuri
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized amino-terminal residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-09 03:59:25	ID requested
⏵ 1	2019-11-11 04:31:43	announced

Selvan LDN, Danda R, Madugundu AK, Puttamallesh VN, Sathe GJ, Krishnan UM, Khetan V, Rishi P, Prasad TSK, Pandey A, Krishnakumar S, Gowda H, Elchuri SV, Phosphoproteomics of Retinoblastoma: A Pilot Study Identifies Aberrant Kinases. Molecules, 23(6):(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: retinoblastoma, phosphoproteomics, LC-MS

Sailaja Elchuri
contact affiliation	Dr. Sailaja V Elchuri,Department of Nanotechnology, VisionResearch Foundation, Sankara Nethralaya, 18-college Road, Nungambakkam, Chennai 600006 India
contact email	sailaja.elchuri@gmail.com
lab head
Sailaja Elchuri
contact affiliation	Sankara Nethralaya
contact email	sailaja.elchuri@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD007228

PRIDE project URI

• PRIDE
  1. PXD007228
     1. Label: PRIDE project
     2. Name: QUANTITATIVE PHOSPHOPROTEOMIC PROFILING OF PRIMARY RETINOBLASTOMA TUMORS