PXD007220 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DSS Microbiota LC-MS/MS . |
Description | In the DSS-induced colitis model, the epithelial damage and resulting inflammation is restricted to the colon, with a potential influence on the microbial composition in the adjacent cecum. Several studies have reported changes of the gut microbiota in the DSS-induced colitis model and other mouse models of IBD. Furthermore, metaproteomics analysis of the gut microbiome in a mouse model of Crohn’s disease demonstrated that disease severity and location are microbiota-dependent, with clear evidence for the causal role of bacterial dysbiosis in the development of chronic ileal inflammation. We have developed a refined model of chronic DSS-induced colitis that reflects typical symptoms of human IBD without a risky body weight loss usually observed in DSS models [Hoffmann et al., submitted]. In this study, we used metaproteomics to characterize the disease-related changes in bacterial protein abundance and function in the refined model of DSS-induced colitis. To assess the structural and functional changes, we applied 16S rRNA gene sequencing and metaproteomics analysis of the intestinal microbiota in three different entities of the intestinal environment, i.e. colon mucus, colon content and cecum content. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_10:08:57.507.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nico Jehmlich |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-08-08 11:12:48 | ID requested | |
⏵ 1 | 2024-10-07 10:08:58 | announced | |
Publication List
10.1021/acs.jproteome.8b00974; |
Haange SB, Jehmlich N, Hoffmann M, Weber K, Lehmann J, von Bergen M, Slanina U, Disease Development Is Accompanied by Changes in Bacterial Protein Abundance and Functions in a Refined Model of Dextran Sulfate Sodium (DSS)-Induced Colitis. J Proteome Res, 18(4):1774-1786(2019) [pubmed] |
Keyword List
curator keyword: Metaproteomics |
submitter keyword: Dextran sulfate sodium (DSS) |
colitis |
mouse model |
metaproteomics |
microbiota |
Contact List
Nico Jehmlich |
contact affiliation | Department Molekulare Systembiologie Helmholtz-Zentrum für Umweltforschung GmbH - UFZ Permoserstraße 15 | 04318 Leipzig |
contact email | nico.jehmlich@ufz.de |
lab head | |
Nico Jehmlich |
contact affiliation | Helmholtz-Centre for Environmental Research - UFZ |
contact email | nico.jehmlich@ufz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007220
- Label: PRIDE project
- Name: DSS Microbiota LC-MS/MS