PXD007207

PXD007207 is an original dataset announced via ProteomeXchange.

Title	Carbene footprinting reveals binding interfaces of a multimeric membrane spanning protein
Description	Mapping the interaction sites between membrane spanning proteins is a key challenge in structural biology. Here we develop and apply carbene footprinting technology to identify the interfacial sites of a trimeric membrane protein. We show how the footprinting probe is effectively incorporated into detergent micelles leading to efficient labelling of the external membrane-spanning regions of the protein.
HostingRepository	PRIDE
AnnounceDate	2017-09-29
AnnouncementXML	Submission_2017-10-04_00:59:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lucio Manzi
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-07 03:41:51	ID requested
1	2017-09-29 09:09:02	announced
⏵ 2	2017-10-04 00:59:51	announced	Updated publication reference for PubMed record(s): 28960650.

Manzi L, Barrow AS, Hopper JTS, Kaminska R, Kleanthous C, Robinson CV, Moses JE, Oldham NJ, Carbene Footprinting Reveals Binding Interfaces of a Multimeric Membrane-Spanning Protein. Angew Chem Int Ed Engl, 56(47):14873-14877(2017) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Carbene, Footprinting, Membrane, Protein, LC-MS

Neil J. Oldham
contact affiliation	University of Nottingham
contact email	neil.oldham@nottingham.ac.uk
lab head
Lucio Manzi
contact affiliation	University of Nottingham
contact email	pcxlm5@nottingham.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD007207

PRIDE project URI

• PRIDE
  1. PXD007207
     1. Label: PRIDE project
     2. Name: Carbene footprinting reveals binding interfaces of a multimeric membrane spanning protein