PXD007184 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The proteomic landscape of patient-derived CD4+ T cells in recent-onset type 1 diabetes |
Description | The pathophysiology underlying the autoimmune disease type 1 diabetes (T1D) is poorly understood. Obtaining an accurate proteomic profile of the T helper cell population is essential for understanding the pathogenesis of T1D. Here, we performed in-depth proteomic profiling of peripheral CD4+ T cells in a pediatric cohort in order to identify cellular signatures associated with the onset of T1D. Using only 250,000 CD4+ T cells per patient, isolated from biobanked PBMC samples, we identified nearly 6,000 proteins using deep-proteome profiling with LC-MS/MS data-independent acquisition. Our analysis revealed an inflammatory signature in patients with T1D; this signature is characterized by circulating mediators of neutrophils, platelets, and the complement system. This signature likely reflects the inflammatory extracellular milieu, suggesting that activation of the innate immune system plays an important role in disease onset. Our results emphasize the potential value of using high-resolution LC-MS/MS to investigate limited quantities of biobanked samples in order to identify disease-relevant proteomic patterns. |
HostingRepository | PRIDE |
AnnounceDate | 2017-12-06 |
AnnouncementXML | Submission_2017-12-06_07:45:16.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marlen Lepper |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-08-03 02:18:48 | ID requested | |
⏵ 1 | 2017-12-06 07:45:17 | announced | |
Publication List
Lepper MF, Ohmayer U, von Toerne C, Maison N, Ziegler AG, Hauck SM, Proteomic Landscape of Patient-Derived CD4+ T Cells in Recent-Onset Type 1 Diabetes. J Proteome Res, 17(1):618-634(2018) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: CD4+ T cells, type 1 diabetes, autoimmune, LC-MS/MS, data-independent, inflammation, biomarker, neutrophil |
Contact List
Stefanie M. Hauck |
contact affiliation | Research Unit Protein Science, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 Neuherberg, Germany |
contact email | hauck@helmholtz-muenchen.de |
lab head | |
Marlen Lepper |
contact affiliation | Research Unit Protein Science, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH) |
contact email | marlen.lepper@helmholtz-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007184
- Label: PRIDE project
- Name: The proteomic landscape of patient-derived CD4+ T cells in recent-onset type 1 diabetes