PXD007183

PXD007183 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Metronidazole Resistance in three seminal lines of Giardia duodenalis
Description	Metronidazole (Mtz) is the frontline chemotherapeutic for a variety of anaerobic pathogens, including the gastroenterinal parasitic protist, Giardia duodenalis. However, treatment failure and clinical resistance is common and increasing. In Giardia, in vitro drug-resistant lines allow controlled experimental interrogation of resistance mechanisms in in vitro, isogenic cultures. These mechanisms centrally involve regulation of glycolytic and antioxidant oxidoreducatases, however this is inconsistent across isolate lines, likely incomplete, and further complicated by phenotypic plasticity in Giardia, which regains Mtz susceptibility after cessation of drug selection, or via passage through the life cycle. Global, quantitative approaches are required to reconstruct complex Mtz resistance phenotypes, resolve isolate variability, and explore unresolved hypotheses regarding post-translational modifications as the regulators of this phenotype. Our study addresses these gaps by comprehensively characterising proteomic changes in three seminal Mtz resistant lines compared to their isogenic, Mtz-susceptible, parental lines. Using quantitative proteomics, we have calculated differentially expressed proteins (DEPs) in MtzR lines, and probed via Western blot changes in protein acetylation, methylation, ubiquitination and phosphorylation post-translational modifications (PTMs). Finally, we have also performed the first controlled, longitudinal study
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:00.980.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samantha Emery
SpeciesList	scientific name: Giardia lamblia ATCC 50803; NCBI TaxID: 184922;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-03 02:17:50	ID requested
1	2018-02-21 07:45:22	announced
2	2018-04-26 01:56:18	announced	Updated publication reference for PubMed record(s): 29688452.
⏵ 3	2024-10-22 04:40:09	announced	2024-10-22: Updated project metadata.

Emery SJ, Baker L, Ansell BRE, Mirzaei M, Haynes PA, McConville MJ, Sv, ä, rd SG, Jex AR, Differential protein expression and post-translational modifications in metronidazole-resistant Giardia duodenalis. Gigascience, 7(4):(2018) [pubmed]

10.1093/gigascience/giy024;

curator keyword: Biological

submitter keyword: proteomics, TMT labelling,Giardia

Aaron Jex
contact affiliation	Population Health and Immunity, Walter and Eliza Hall Institute of Medical Research
contact email	jex.a@wehi.edu.au
lab head
Samantha Emery
contact affiliation	Population Health and Immunity, Walter and Eliza Hall Institute of Medical Research
contact email	emery.s@wehi.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007183
     1. Label: PRIDE project
     2. Name: Proteomics of Metronidazole Resistance in three seminal lines of Giardia duodenalis