PXD007182

PXD007182 is an original dataset announced via ProteomeXchange.

Title	Tankyrase modulates insulin sensitivity in skeletal muscle cells by regulating stability of GLUT4 vesicle proteins
Description	Tankyrase1 and 2, members of the poly(ADP-ribose) polymerase family, play a role in multiple biological processes including Wnt signalling and glucose uptake. However, their role in glucose uptake remains elusive, particularly in skeletal muscle cells. Treatment of differentiated L6 myotubes with the small molecule tankyrase inhibitor XAV939 resulted in insulin resistance as determined by impaired insulin-stimulated glucose uptake. Proteomic analysis identified 109 proteins that were down-regulated and 109 proteins that were up-regulated in response to XAV939. Among the down-regulated proteins there were several glucose transporter GLUT4 storage vesicle (GSV) proteins including RAB10, VAMP8, SORT1 as well as GLUT4 itself. Knock down of tankyrase1 in L6 myotubes also led to a reduction in the level of GSV proteins and impaired insulin-mediated glucose uptake. Inhibition of the proteasome rescued protein levels of GSV proteins as well as insulin-stimulated glucose uptake in XAV939-treated L6 myotubes. These studies reveal an important role for tankyrase in maintaining the stability of key GLUT4 regulatory proteins that in turn plays a role in regulating cellular insulin sensitivity.
HostingRepository	PRIDE
AnnounceDate	2018-04-17
AnnouncementXML	Submission_2019-02-12_08:36:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiduan Su
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-03 02:13:05	ID requested
1	2018-04-17 00:33:09	announced
⏵ 2	2019-02-12 08:36:52	announced	Updated publication reference for PubMed record(s): 29669812.

Su Z, Deshpande V, James DE, St, ö, ckli J, Tankyrase modulates insulin sensitivity in skeletal muscle cells by regulating the stability of GLUT4 vesicle proteins. J Biol Chem, 293(22):8578-8587(2018) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Tankyrase, GSV, Skeletal muscle

David James
contact affiliation	Leonard P Ullmann Chair in Metabolic Systems Biology, School of Life and Environmental Sciences and Sydney Medical School D17 - Charles Perkins Centre | The University of Sydney | NSW | 2006
contact email	david.james@sydney.edu.au
lab head
Zhiduan Su
contact affiliation	The University of Sydney
contact email	zhiduan.su@sydney.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007182
     1. Label: PRIDE project
     2. Name: Tankyrase modulates insulin sensitivity in skeletal muscle cells by regulating stability of GLUT4 vesicle proteins