PXD007180

PXD007180 is an original dataset announced via ProteomeXchange.

Title	Cigarette smoke alters the secretome of lung epithelial cells
Description	Cigarette smoke is the most relevant risk factor for the development of lung cancer and chronic obstructive pulmonary disease. Many of its more than 4500 chemicals are highly reactive, thereby altering protein structure and function. Here, we used subcellular fractionation coupled to label-free quantitative MS to globally assess alterations in the proteome of different compartments of lung epithelial cells upon exposure to cigarette smoke extract. Proteomic profiling of the human alveolar derived cell line A549 revealed the most pronounced changes within the cellular secretome with preferential downregulation of proteins involved in wound healing and extracellular matrix organization. In particular, secretion of secreted protein acidic and rich in cysteine, a matricellular protein that functions in tissue response to injury, was consistently diminished by cigarette smoke extract in various pulmonary epithelial cell lines and primary cells of human and mouse origin as well as in mouse ex vivo lung tissue cultures. Our study reveals a previously unrecognized acute response of lung epithelial cells to cigarette smoke that includes altered secretion of proteins involved in extracellular matrix organization and wound healing. This may contribute to sustained alterations in tissue remodeling as observed in lung cancer and chronic obstructive pulmonary disease.
HostingRepository	PRIDE
AnnounceDate	2019-01-08
AnnouncementXML	Submission_2019-01-08_04:28:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliane Merl-Pham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-02 09:53:22	ID requested
1	2019-01-08 04:17:38	announced
⏵ 2	2019-01-08 04:28:22	announced	Updated publication reference for PubMed record(s): 27891773, 30557664.

Kammerl IE, Caniard A, Merl-Pham J, Ben-Nissan G, Mayr CH, Mossina A, Geerlof A, Eickelberg O, Hauck SM, Sharon M, Meiners S, Dissecting the molecular effects of cigarette smoke on proteasome function. J Proteomics, 193():1-9(2019) [pubmed]

Mossina A, Lukas C, Merl-Pham J, Uhl FE, Mutze K, Schamberger A, Staab-Weijnitz C, Jia J, Yildirim A Ö, K, ö, nigshoff M, Hauck SM, Eickelberg O, Meiners S, Cigarette smoke alters the secretome of lung epithelial cells. Proteomics, 17(1-2):(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Cigarette smoke, lung, proteomic profiling, secretome

Silke Meiners
contact affiliation	Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany
contact email	silke.meiners@helmholtz-muenchen.de
lab head
Juliane Merl-Pham
contact affiliation	Research Unit Protein Science, Helmholtz Center Munich
contact email	juliane.merl@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD007180

PRIDE project URI

• PRIDE
  1. PXD007180
     1. Label: PRIDE project
     2. Name: Cigarette smoke alters the secretome of lung epithelial cells