PXD007176

PXD007176 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of L6 myoblasts treated with C-terminal transgelin peptides
Description	To investigate bioactivity of peptides identified in human plasma, we treated L6 myoblasts in vitro with a synthetic C-terminal peptide from TAGLN spanning amino acids 179-200, or a synthetic C-terminal peptide from TAGLN2 spanning amino acids 178-199 (1 µg/mL; 7 days), and compared the treatments to control myoblasts cultured for the same amount of time with the addition of only PBS. Proteomic analysis by single-shot nanoUHPLC-MS/MS was performed on four biological replicates and quantification performed by LFQ. A total of 6,095 proteins were identified with 4,710 quantified in at least three biological replicates (Supplemental Table S4). A total of 222 and 307 proteins were regulated by the TAGLN and TAGLN2 peptides relative to the control treatments, respectively, with 61 proteins co-regulated by both TAGLN and TAGLN2 (P<0.05; two-sample t-test with Benjamini-Hochberg correction) (Figure 3G).
HostingRepository	PRIDE
AnnounceDate	2017-11-21
AnnouncementXML	Submission_2017-11-21_05:13:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Parker
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-02 06:52:40	ID requested
⏵ 1	2017-11-21 05:13:54	announced

Parker BL, Burchfield JG, Clayton D, Geddes TA, Payne RJ, Kiens B, Wojtaszewski JFP, Richter EA, James DE, Multiplexed Temporal Quantification of the Exercise-regulated Plasma Peptidome. Mol Cell Proteomics, 16(12):2055-2068(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: myoblasts, peptidomics, proteomics

Benjamin Parker
contact affiliation	The University of Sydney
contact email	benjamin.parker@sydney.edu.au
lab head
Benjamin Parker
contact affiliation	The University of Sydney
contact email	benjamin.parker@sydney.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007176
     1. Label: PRIDE project
     2. Name: Proteomic analysis of L6 myoblasts treated with C-terminal transgelin peptides