PXD007175

PXD007175 is an original dataset announced via ProteomeXchange.

Title	Oxidative stress-triggered interactions between the succinyl- and acetyl-proteomes of rice leaves
Description	Protein lysine acylation, such as succinylation and acetylation are important posttranslational modification (PTM) mechanisms, with key roles in the regulation of diverse metabolic and cellular processes. Antibody-based affinity enrichment, high-resolution LC-MS/MS analysis and integrated bioinformatics analysis, were used to characterize the lysine succinylome (Ksuc) and acetylome (Kace) in rice leaves. Rice (Oryza sativa., Wuyunjing 7) was kindly provided byJiangsu Academy of Agricultural Sciences, Jiangsu Province, China. Rice seeds were surface-sterilized with 5% NaClO for 20 min, washed extensively with distilled water and then germinated in distilled water at 28ºC for 2 days. Germinated seeds were cultivated on half-strength ammonium-free Murashige and Skoog (MS) medium. For oxidative stress treatments, 14-days-old rice seedlings were transferred to 1/2 MS medium in the absence or presence of 1 mM H2O2 for 6 days. Rice seedlings were then harvested for protein extraction.
HostingRepository	PRIDE
AnnounceDate	2018-07-23
AnnouncementXML	Submission_2018-07-23_07:34:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Iris Finkemeier
SpeciesList	scientific name: Rice(O. sativa); NCBI TaxID: 4530;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; N6-succinyl-L-lysine; monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-02 06:43:16	ID requested
⏵ 1	2018-07-23 07:34:20	announced

Zhou H, Finkemeier I, Guan W, Tossounian MA, Wei B, Young D, Huang J, Messens J, Yang X, Zhu J, Wilson MH, Shen W, Xie Y, Foyer CH, Oxidative stress-triggered interactions between the succinyl- and acetyl-proteomes of rice leaves. Plant Cell Environ, 41(5):1139-1153(2018) [pubmed]

curator keyword: Biological

submitter keyword: Rice, hydrogen peroxide, lysine acetylation, lysine succinylation, proteome

Prof. Iris Finkemeier
contact affiliation	Institute of Plant Biology and Biotechnology University of Muenster Schlossplatz 7 48149 Muenster Germany
contact email	iris.finkemeier@uni-muenster.de
lab head
Iris Finkemeier
contact affiliation	University of Muenster
contact email	iris.finkemeier@uni-muenster.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007175
     1. Label: PRIDE project
     2. Name: Oxidative stress-triggered interactions between the succinyl- and acetyl-proteomes of rice leaves