PXD007170

PXD007170 is an original dataset announced via ProteomeXchange.

Title	Human Salivary Cystatins - SALIVARY CYSTATINS: EXPLORING NEW POST-TRANSLATIONAL MODIFICATIONS AND POLYMORPHISMS BY TOP-DOWN HIGH RESOLUTION MASS SPECTROMETRY
Description	In the present study various proteoforms of cystatin A, cystatin B, cystatin D, cystatin S, cystatin SN and cystatin SA were detected and characterized by a top-down HPLC-ESI-MS platform integrated by bottom-up strategies in the acidic soluble fraction of human saliva. The several proteoforms derive from polymorphisms of the coding sequence and post-translational modifications, typically phosphorylation, N-terminal processing, and oxidation. The protein species detected in saliva collected from healthy subjects were: (i) cystatin AT96→M and its acetylated derivative; (ii) cystatin B N-terminally acetylated and carboxymethylated at C3; (iii) N-terminally truncated cystatin D with the N-terminal Q converted to pyro-E and lacking the first 5 amino acid residues (pGlu-cystatin D Des1-5); (iv) N-terminally truncated forms of cystatin SN and SNP11→L lacking the first 4 amino acids (cystatin SN Des1-4 and cystatin SNP11→L Des1-4) and the first 7 amino acids (cystatin SN Des1-7 and cystatin SNP11→L Des1-7); (v) N-terminally truncated cystatin SA lacking the first 7 amino acids (cystatin SA Des1-7); (vi) oxidized derivatives of cystatins SN and S1 at W23 and W107.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:56.855.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Manconi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetic acid derivatized residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-02 02:51:29	ID requested
1	2017-10-13 00:21:15	announced
⏵ 2	2024-10-22 04:40:05	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.7b00567;

Manconi B, Liori B, Cabras T, Vincenzoni F, Iavarone F, Castagnola M, Messana I, Olianas A, Salivary Cystatins: Exploring New Post-Translational Modifications and Polymorphisms by Top-Down High-Resolution Mass Spectrometry. J Proteome Res, 16(11):4196-4207(2017) [pubmed]

curator keyword: Biological

submitter keyword: S-type Cystatins, post-translational modifications,Cystatin A, top-down high-resolution mass spectrometry., Cystatin D, Cystatin B, human saliva

Barbara Manconi
contact affiliation	Department of Life and Environmental Sciences, Biomedical Section, University of Cagliari, Monserrato, CA, Italy.
contact email	bmanconi@unica.it
lab head
Barbara Manconi
contact affiliation	University of Cagliari
contact email	bmanconi@unica.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD007170

PRIDE project URI

• PRIDE
  1. PXD007170
     1. Label: PRIDE project
     2. Name: Human Salivary Cystatins - SALIVARY CYSTATINS: EXPLORING NEW POST-TRANSLATIONAL MODIFICATIONS AND POLYMORPHISMS BY TOP-DOWN HIGH RESOLUTION MASS SPECTROMETRY