PXD007165

PXD007165 is an original dataset announced via ProteomeXchange.

Title	EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification
Description	We developed EASI-tag (Easily Abstractable Sulfoxide-based Isobaric tag), a new generation of amine-derivatizing and sulfoxide-containing isobaric labelling reagents, which dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of 12C precursors and quantification at the MS2 level enable accurate determination of quantitative differences between multiplexed samples. EASI-tag makes isobaric labeling applicable to any bottom up proteomics application and to benchtop mass spectrometers.
HostingRepository	PRIDE
AnnounceDate	2018-06-21
AnnouncementXML	Submission_2018-06-21_00:04:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastian Virreira Winter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-01 05:56:57	ID requested
⏵ 1	2018-06-21 00:04:46	announced

Virreira Winter S, Meier F, Wichmann C, Cox J, Mann M, Meissner F, EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification. Nat Methods, 15(7):527-530(2018) [pubmed]

curator keyword: Technical

submitter keyword: mass spectrometry, isobaric labeling, proteomics

Matthias Mann
contact affiliation	Max Planck Institute of Biochemistry
contact email	mmann@biochem.mpg.de
lab head
Sebastian Virreira Winter
contact affiliation	Max Planck Institute of Biochemistry
contact email	virreira@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD007165

PRIDE project URI

• PRIDE
  1. PXD007165
     1. Label: PRIDE project
     2. Name: EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification