PXD007164

PXD007164 is an original dataset announced via ProteomeXchange.

Title	Correlation of isolate-specific oxidative stress responses at the protein level in Aspergillus flavus with aflatoxin production capability
Description	Aspergillus flavus is a pathogen of corn, peanut, and other crops which produces carcinogenic mycotoxins known as aflatoxins. Previous studies have shown that drought stress results in exacerbated aflatoxin production. Drought-associated oxidative stress caused by reactive oxygen species (ROS) is suspected to contribute to increased aflatoxin production during infection. Here, the responses of field isolates of A. flavus with varying degrees of aflatoxin production capability to H2O2-derived oxidative stress were examined using iTRAQ proteomics. Three isolates: AF13 (highly toxigenic), NRRL3357 (moderately toxigenic), and K54A (atoxigenic), were cultures in aflatoxin conducive yeast extract-sucrose (YES) medium amended with 0, 10, or 20/25mM of H2O2. Identified differentially expressed proteins were used for functional prediction, cellular localization, and pathway analyses to identify molecular mechanisms involved in A. flavus oxidative stress responses relative to aflatoxin production capability. Correlative analyses with previously obtained transcriptome data for the same isolates under the same experimental conditions was also performed with a low degree of correlation (r = 0.1114) observed between the protein and transcript data suggesting possible post-transcriptional regulation of oxidative stress responses. The identified stress responsive mechanisms provide a basis of investigating novel approaches of enhancing host resistance against aflatoxin contamination.
HostingRepository	PRIDE
AnnounceDate	2018-02-27
AnnouncementXML	Submission_2018-02-27_08:28:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jake Fountain
SpeciesList	scientific name: Aspergillus flavus; NCBI TaxID: 5059;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-01 01:40:45	ID requested
⏵ 1	2018-02-27 08:28:55	announced

Fountain JC, Koh J, Yang L, Pandey MK, Nayak SN, Bajaj P, Zhuang WJ, Chen ZY, Kemerait RC, Lee RD, Chen S, Varshney RK, Guo B, Proteome analysis of Aspergillus flavus isolate-specific responses to oxidative stress in relationship to aflatoxin production capability. Sci Rep, 8(1):3430(2018) [pubmed]

curator keyword: Biological

submitter keyword: Aspergillus flavus, aflatoxin, drought stress, oxidative stress, iTRAQ proteomics

Baozhu Guo
contact affiliation	USDA-ARS Crop Protection and Management Research Unit Tifton, GA 31794
contact email	baozhu.guo@ars.usda.gov
lab head
Jake Fountain
contact affiliation	University of Georgia
contact email	jfount1@uga.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007164
     1. Label: PRIDE project
     2. Name: Correlation of isolate-specific oxidative stress responses at the protein level in Aspergillus flavus with aflatoxin production capability