PXD007162

PXD007162 is an original dataset announced via ProteomeXchange.

Title	The proteome of germlings from urediniospores of Puccinia triticina
Description	Wheat leaf rust is a serious fungal disease of wheat that causes annual losses and necessitates using fungicides for effective disease management. It is caused by Puccinia triticina which spreads by means of airborne urediniospores. When these germinate on the leaf surface, they form germ-tubes which enter the leaf through open stomates. Spores and germ-tubes represent the first fungal structures that the host can perceive during a rust infection. They therefore contain proteins that could potentially trigger early host defense responses. Using 2-DE to separate this proteome, we produced gels containing 173 spots in the pI range of 4-7 and identified 123 proteins. These were predominantly proteins involved in metabolic and cellular processes, but with a large number (77%) of novel proteins that could not be identified through homology matching Twenty four of these showed no homology to wheat sequences, making them good candidate PAMPs.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:10.938.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Rampitsch
SpeciesList	scientific name: Puccinia triticina 1-1 BBBD Race 1; NCBI TaxID: 630390;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-31 07:44:46	ID requested
1	2022-02-28 02:38:14	announced
⏵ 2	2023-11-14 08:49:11	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: wheat leaf rust, brown rust

Christof Rampitsch
contact affiliation	Agriculture and Agrifood Canada Morden Research and Development Centre
contact email	chris.rampitsch@agr.gc.ca
lab head
Christof Rampitsch
contact affiliation	Molecular Genetics
contact email	chris.rampitsch@agr.gc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007162
     1. Label: PRIDE project
     2. Name: The proteome of germlings from urediniospores of Puccinia triticina