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PXD007160

PXD007160 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal quantitative analysis of the human brain proteome in Alzheimer’s and Parkinson’s Disease
DescriptionPatients with Alzheimer’s disease (AD) and Parkinson disease (PD) often have overlap in brain neuropathology and clinical presentation suggesting that these two diseases share common underlying mechanisms. Currently, the molecular events linking AD and PD are incompletely understood. Utilizing 10-plex Tandem Mass Tag (TMT) assays and MultiNotch MS3 mass spectrometry, we performed an unbiased quantitative proteomic analysis of post-mortem human brain tissues (n=80) from four different groups defined as healthy controls, AD, PD, and co-morbid AD/PD cases across two brain regions (frontal cortex and anterior cingulate gyrus). In total, we identified 11,840 protein groups representing 10,230 gene symbols, which map to ~65% of the protein coding genes in brain. The utility of including two reference standards in each TMT 10-plex assay to assess intra-batch and inter-batch variance is also described. Ultimately, this comprehensive human brain proteomic dataset serves as a valuable resource for various research endeavors including, but not limited to, the identification of protein signatures and neuro-centric signaling pathways that are common or distinct in AD and PD.
HostingRepositoryPRIDE
AnnounceDate2018-02-07
AnnouncementXMLSubmission_2019-02-12_08:35:44.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDuc Duong
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-07-31 06:57:02ID requested
12018-02-09 11:43:02announced
22019-02-12 08:35:46announcedUpdated publication reference for PubMed record(s): 29533394.
Publication List
Ping L, Duong DM, Yin L, Gearing M, Lah JJ, Levey AI, Seyfried NT, Global quantitative analysis of the human brain proteome in Alzheimer's and Parkinson's Disease. Sci Data, 5():180036(2018) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Alzheimer's, Parkinson's , Human, Brain, TMT, SPS, LC-MS/MS
Contact List
Nicholas Seyfried
contact affiliationEmory University School of Medicine Biochemistry Department
contact emailnseyfri@emory.edu
lab head
Duc Duong
contact affiliationCenter of Neurodegenerative Diseases
contact emaildduong@emory.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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