PXD007156

PXD007156 is an original dataset announced via ProteomeXchange.

Title	Identification of Long Lived Synaptic Proteins by Comprehensive Proteomic Analysis of Synaptosome Protein Turnover
Description	Memory formation is believed result from changes in synapse strength and structure. While memories may persist for the lifetime of an organism, the proteins and lipids that make up synapses undergo constant turnover with lifetimes from minutes to days. The molecular basis for memory maintenance may rely on a subset of long-lived proteins (LLPs). While it is known that LLPs exist, whether such proteins are present at synapses is unknown. We performed an unbiased screen using metabolic pulse-chase labeling in mice and cultured neurons combined with quantitative proteomics and identified synaptic LLPs with half-lives of several weeks or longer. Synaptic proteins generally exhibited longer lifetimes than cytosolic proteins. Protein turnover was sensitive to pharmacological manipulations of activity in neuronal cultures or in mice exposed to an enriched environment. We show that synapses contain LLPs and that global synapse protein turnover is modulated by neuronal activity and behavioral experience.
HostingRepository	PRIDE
AnnounceDate	2018-03-19
AnnouncementXML	Submission_2018-04-09_00:36:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	chanhyun na
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	6x(13)C labeled residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-31 06:46:25	ID requested
1	2018-03-19 02:16:31	announced
⏵ 2	2018-04-09 00:36:52	announced	Updated publication reference for PubMed record(s): 29610302.

Heo S, Diering GH, Na CH, Nirujogi RS, Bachman JL, Pandey A, Huganir RL, Identification of long-lived synaptic proteins by proteomic analysis of synaptosome protein turnover. Proc Natl Acad Sci U S A, 115(16):E3827-E3836(2018) [pubmed]

curator keyword: Biological

submitter keyword: Long lived protein, Synaptosome, Protein turnover

Richard L. Huganir
contact affiliation	The Solomon H. Snyder Department of Neuroscience Johns Hopkins University School of Medicine USA
contact email	rhuganir@jhmi.edu
lab head
chanhyun na
contact affiliation	Johns Hopkins University
contact email	chanhyun.na@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007156
     1. Label: PRIDE project
     2. Name: Identification of Long Lived Synaptic Proteins by Comprehensive Proteomic Analysis of Synaptosome Protein Turnover