PXD007153

PXD007153 is an original dataset announced via ProteomeXchange.

Title	Systematic and quantitative assessment of hydrogen peroxide reactivity with cysteines across human proteomes
Description	Protein cysteinyl residues are the mediators of hydrogen peroxide (H2O2)-dependent redox signaling. However, site-specific mapping of the selectivity and dynamics of these redox reactions in cells poses a major analytical challenge. Here we describe a chemoproteomic platform to systematically and quantitatively analyze the reactivity of thousands of cysteines toward H2O2 in human cells. We identified >900 H2O2-sensitive cysteines, which are defined as the H2O2-dependent redoxome. Although redox sites associated with antioxidative and metabolic functions are consistent, most of the H2O2-dependent redoxome varies dramatically between different cells. Structural analyses reveal that H2O2-sensitive cysteines are less conserved than their redox-insensitive counterparts and display distinct sequence motifs, structural features, and potential for crosstalk with lysine modifications. Notably, our chemoproteomic platform also provides an opportunity to predict oxidation-triggered protein conformational changes. The data are freely accessible as a resource at http://redox.ncpsb.org/OXID/.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:05.112.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007153
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jing Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl; unknown modification
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-31 01:30:12	ID requested
1	2017-08-25 01:41:05	announced
⏵ 2	2024-10-22 04:40:06	announced	2024-10-22: Updated project metadata.

Fu L, Liu K, Sun M, Tian C, Sun R, Morales Betanzos C, Tallman KA, Porter NA, Yang Y, Guo D, Liebler DC, Yang J, Systematic and Quantitative Assessment of Hydrogen Peroxide Reactivity With Cysteines Across Human Proteomes. Mol Cell Proteomics, 16(10):1815-1828(2017) [pubmed]

10.6019/PXD007153;

10.1074/mcp.ra117.000108;

curator keyword: Biological

submitter keyword: Cysteine;Chemoproteomics

yang jing
contact affiliation	National Center for Protein Sciences Beijing
contact email	yangjing54@hotmail.com
lab head
Jing Yang
contact affiliation	National Center for Protein Sciences, Beijing
contact email	yangjing54@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007153
     1. Label: PRIDE project
     2. Name: Systematic and quantitative assessment of hydrogen peroxide reactivity with cysteines across human proteomes