PXD007146 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | INA complex liaises the F1Fo-ATP synthase membrane motor modules - Atp23 pulldown dataset |
Description | The F1Fo-ATP synthase translates a proton flux across the inner mitochondrial membrane into a mechanical rotation, driving anhydride bond formation in the catalytic portion. The complex's membrane-embedded motor section forms a proteinaceous channel at the interface between Atp9 ring and Atp6. To prevent unrestricted proton flow dissipating the H+-gradient, channel formation is a critical and tightly controlled step during ATP synthase assembly. Here we show that the INA complex (INAC) acts at this decisive step promoting Atp9 ring association with Atp6. INAC binds initially to newly synthesized mitochondrial-encoded Atp6 and Atp8 in complex with maturation factors. INAC association is retained until the F1-portion is built on Atp6/8 and loss of INAC causes accumulation of the free F1. An independent complex is formed between INAC and the Atp9 ring. We conclude that INAC maintains assembly intermediates of the F1Fo-ATP synthase in a primed state for the terminal assembly step – channel and motor module formation. |
HostingRepository | PRIDE |
AnnounceDate | 2017-11-03 |
AnnouncementXML | Submission_2017-11-03_08:37:14.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Friedel Drepper |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-07-27 10:27:50 | ID requested | |
⏵ 1 | 2017-11-03 08:37:15 | announced | |
Publication List
Naumenko N, Morgenstern M, Ruckt, ä, schel R, Warscheid B, Rehling P, -ATP synthase membrane motor modules. Nat Commun, 8(1):1237(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Saccharomyces cerevisiae, interaction proteomics |
Contact List
Prof. Dr. Bettina Warscheid |
contact affiliation | Department of Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology and BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg im Breisgau, Germany |
contact email | Bettina.Warscheid@biologie.uni-freiburg.de |
lab head | |
Friedel Drepper |
contact affiliation | AG Warscheid Biologie II Albert-Ludwigs-Universität Freiburg Schänzlestr. 1 79104 Freiburg Germany |
contact email | friedel.drepper@biologie.uni-freiburg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007146
- Label: PRIDE project
- Name: INA complex liaises the F1Fo-ATP synthase membrane motor modules - Atp23 pulldown dataset