PXD007130

PXD007130 is an original dataset announced via ProteomeXchange.

Title	Microenvironment remodeling during human iPSC-derived NSC 3D differentiation
Description	Brain microenvironment plays an important role in neurodevelopment and function, where extracellular matrix (ECM) components and soluble factors modulate cellular features, as migration, proliferation survival and neuronal function. Disruption of microenvironment’s homeostasis is often related to pathological conditions. Here, we addressed the microenvironment remodeling occurring during in vitro differentiation of human neural stem cells (NSC) in a three-dimensional (3D) culture system. Proteome and transcriptome dynamics revealed significant changes namely at cell membrane and ECM composition during 3D differentiation, diverging significantly from the profile of monolayer cultures (2D). Structural proteoglycans typically found in brain ECM were enriched during 3D differentiation, while 2D cultures presented increased levels of basement membrane constituents (e.g., laminins, collagens and fibrillins). Moreover, higher expression levels of synaptic machinery and ion transport machinery constituents observed for 3D cultures, both at mRNA and protein levels, suggested a higher degree of neuronal maturation and organization relative to 2D differentiation. This work demonstrated that neural cellular and extracellular features can be recapitulated in the presented 3D neural cell model, highlighting its value to address molecular defects in cell-ECM interactions associated with neurological disorders. Associated GEO dataset is available at GSE102139
HostingRepository	PRIDE
AnnounceDate	2018-09-06
AnnouncementXML	Submission_2018-09-06_02:20:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Simão
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-26 01:41:34	ID requested
1	2018-07-31 08:23:49	announced
⏵ 2	2018-09-06 02:20:32	announced	Updated project metadata.

Sim, ã, o D, Silva MM, Terrasso AP, Arez F, Sousa MFQ, Mehrjardi NZ, Š, ari, ć T, Gomes-Alves P, Raimundo N, Alves PM, Brito C, Recapitulation of Human Neural Microenvironment Signatures in iPSC-Derived NPC 3D Differentiation. Stem Cell Reports, 11(2):552-564(2018) [pubmed]

curator keyword: Biological

submitter keyword: 3D cultures

Neual differentiation

hiPSC-derived NSC

ECM

Microenvironment

SWATH

Catarina Brito
contact affiliation	iBET, Instituto de Biologia Experimental e Biológica, Oeiras, Portugal Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal
contact email	anabrito@itqb.unl.pt
lab head
Daniel Simão
contact affiliation	iBET, Instituto de Biologia Experimental e Biológica, Oeiras, Portugal; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal
contact email	dsimao@itqb.unl.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD007130

PRIDE project URI

• PRIDE
  1. PXD007130
     1. Label: PRIDE project
     2. Name: Microenvironment remodeling during human iPSC-derived NSC 3D differentiation