PXD007127

PXD007127 is an original dataset announced via ProteomeXchange.

Title	Molecular alterations elicited by chronic exposure to cigarette smoke and chewing tobacco in oral keratinocytes
Description	Tobacco is mainly consumed in two different forms (smoking and chewing) that vary in their composition and methods of intake. Despite being the leading cause of oral cancer, the molecular mechanisms resulting in malignancy upon tobacco exposure are yet to be fully elucidated. We therefore sought to compare the molecular alterations in oral keratinocytes exposed to smoke and chewing tobacco. OKF6/TERT1 cells were exposed to cigarette smoke condensate or chewing tobacco for progressively increasing durations (2, 4, 6 and 8 months). We employed a TMT-based quantitative proteomics approach to investigate the adverse effects of chronic cigarette smoke or chewing tobacco exposure in oral keratinocytes. LC/MS3 analysis resulted in the quantification of 5,342 proteins and 2,821 proteins in cigarette smoke and chewing tobacco exposed cells, respectively. Upstream regulator analysis indicates the involvement of distinct regulators in CSC exposed cells compared to STE exposed cells. In addition, exome sequencing revealed discrete genetic alterations in cells exposed to each insult. Current analysis defines a clear distinction in the molecular dysregulation in oral cells in response to different tobacco-based insults. Some of the proteins dysregulated in cigarette smoke or chewing tobacco exposed cells may serve as potential early detection biomarkers which could aid in stratification of patients based on tobacco usage history.
HostingRepository	PRIDE
AnnounceDate	2019-11-08
AnnouncementXML	Submission_2019-11-08_09:15:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aditi Chatterjee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-25 07:51:38	ID requested
⏵ 1	2019-11-08 09:15:14	announced

Rajagopalan P, Patel K, Jain AP, Nanjappa V, Datta KK, Subbannayya T, Mangalaparthi KK, Kumari A, Manoharan M, Coral K, Murugan S, Nair B, Prasad TSK, Mathur PP, Gupta R, Gupta R, Khanna-Gupta A, Califano J, Sidransky D, Gowda H, Chatterjee A, Molecular alterations associated with chronic exposure to cigarette smoke and chewing tobacco in normal oral keratinocytes. Cancer Biol Ther, 19(9):773-785(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Orbitrap Fusion, high-throughput, carcinogenesis, smoking, chronic exposure

Aditi Chatterjee
contact affiliation	Institute of Bioinformatics, Unit 1, 7th floor, Discoverer building, ITPL, Whitefield, Bangalore - 560066, Karnataka, India
contact email	aditixchatterjee@gmail.com
lab head
Aditi Chatterjee
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India
contact email	aditixchatterjee@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007127
     1. Label: PRIDE project
     2. Name: Molecular alterations elicited by chronic exposure to cigarette smoke and chewing tobacco in oral keratinocytes