PXD007126

PXD007126 is an original dataset announced via ProteomeXchange.

Title	Identification of a precursor processing protease from the spider Cupiennius salei essential for venom neurotoxin maturation
Description	Spider venom neurotoxins and cytolytic peptides are expressed as elongated precursor peptides, which are post-translationally processed by proteases to yield the active mature peptides. The recognition motifs for these processing proteases, first published more than ten years ago, include the Processing Quadruplet Motif (PQM) and the inverted Processing Quadruplet Motif (iPQM). However, the identification of the relevant proteases was still pending. Here we describe the purification of a neurotoxin precursor processing protease from the venom of the spider Cupiennius salei. The chymotrypsin – like serine protease is a 28 kDa heterodimer with optimum activity at venom’s pH of 6.0. We designed multiple synthetic peptides mimicking the predicted cleavage sites of neurotoxin precursors. Using these peptides as substrates, we confirm the biochemical activity of the protease in propeptide removal from neurotoxin precursors by cleavage C-terminal of the PQM. Furthermore, the PQM protease also cleaves the iPQM relevant for heterodimerization of a subgroup of neurotoxins. An involvement in the maturing of cytolytic peptides is very likely, due to high similarity of present protease recognition motifs. Finally, bioinformatics analysis, identifying sequences of homolog proteins from 18 spiders of 9 families, demonstrate the wide distribution and importance of the isolated enzyme for spiders. In summary, we establish the first example of a PQM protease, essential for maturing of spider venom neurotoxins. In the future, the here described protease may be established as powerful tool for production strategies of recombinant toxic peptides, adapted to the maturing of spider venom toxins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:21.862.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Langenegger
SpeciesList	scientific name: Cupiennius salei (American wandering spider); NCBI TaxID: 6928;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-25 06:42:59	ID requested
1	2018-01-02 02:53:09	announced
⏵ 2	2024-10-22 04:39:30	announced	2024-10-22: Updated project metadata.

10.1074/jbc.m117.810911;

Langenegger N, Koua D, Sch, ü, rch S, Heller M, Nentwig W, Kuhn-Nentwig L, essential for venom neurotoxin maturation. J Biol Chem, 293(6):2079-2090(2018) [pubmed]

curator keyword: Biological

submitter keyword: iPQM, neurotoxin, PQM, propeptide, venom,Cupiennius salei, processing quadruplet motif, protease, enzyme purification

Lucia Kuhn-Nentwig
contact affiliation	Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, CH-3012 Bern, Switzerland
contact email	lucia.kuhn@iee.unibe.ch
lab head
Nicolas Langenegger
contact affiliation	Institute of Ecology and Evolution, University of Bern, Switzerland
contact email	nicolas.langenegger@iee.unibe.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD007126

PRIDE project URI

• PRIDE
  1. PXD007126
     1. Label: PRIDE project
     2. Name: Identification of a precursor processing protease from the spider Cupiennius salei essential for venom neurotoxin maturation