PXD007114

PXD007114 is an original dataset announced via ProteomeXchange.

Title	The RNA Polymerase II factor RPAP1 is critical for Mediator-driven transcription and cell identity
Description	The RNA Polymerase II Associated Protein 1 (RPAP1) is conserved across metazoa and critical for stem cell differentiation in plants, however, very little is known about its mechanism of action or its role in mammalian cells. Here, we report that RPAP1 is essential for the expression of lineage specifying factors and for viability. Accordingly, inhibition of RPAP1 triggers somatic cell de-differentiation and facilitates reprogramming into pluripotent stem cells. Conversely, interfering with RPAP1 in ES cells severely impairs their differentiation capacity. Mechanistically, we show that RPAP1 is essential for the interaction between Pol II and Mediator, as well as for the recruitment of important regulators, such as the Mediator-specific Pol II factor POLR2M/Gdown1 and the CTD phosphatase RPAP2. In agreement, depletion of RPAP1 disturbs the loading of Pol II and Pol II Ser5 phosphorylation levels and impairs expression of super-enhancer-driven genes. We conclude that Mediator-RPAP1-Pol II is an ancient module, conserved from plants to mammals, critical for establishing and maintaining cell identity.
HostingRepository	PRIDE
AnnounceDate	2018-01-10
AnnouncementXML	Submission_2019-02-12_08:34:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nuria Ibarz
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-25 01:00:16	ID requested
1	2018-01-10 00:09:13	announced
⏵ 2	2019-02-12 08:34:36	announced	Updated publication reference for PubMed record(s): 29320736.

Lynch CJ, Bernad R, Calvo I, N, ó, brega-Pereira S, Ruiz S, Ibarz N, Martinez-Val A, Gra, ñ, a-Castro O, G, ó, mez-L, ó, pez G, Andr, é, s-Le, ó, n E, Espinosa Angarica V, Del Sol A, Ortega S, Fernandez-Capetillo O, Rojo E, Munoz J, Serrano M, The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity. Cell Rep, 22(2):396-410(2018) [pubmed]

curator keyword: Biological

submitter keyword: RPAP1, RNA pol II, mediator, cell identity

Javier Munoz
contact affiliation	CNIO
contact email	jmunozpe@cnio.es
lab head
Nuria Ibarz
contact affiliation	Cnio
contact email	nibarz@cnio.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD007114

PRIDE project URI

• PRIDE
  1. PXD007114
     1. Label: PRIDE project
     2. Name: The RNA Polymerase II factor RPAP1 is critical for Mediator-driven transcription and cell identity