PXD007085

PXD007085 is an original dataset announced via ProteomeXchange.

Title	An immunoproteomic approach to characterize the CAR interactome and signalosome
Description	Adoptive transfer of chimeric antigen receptor (CAR)-T cells is expected to become the first line of treatment for multiple malignancies, following the enormous success of anti-CD19 therapies. However, their mechanism of action is not fully understood, and clear guidelines for the design of safe and efficient receptors are missing. We hereby describe a systematic analysis of the CAR “interactome” in human primary T cells, which allowed us to identify molecular traits that influence CAR-T cell efficacy. Interactome analysis was based on immunoprecipitation of CARs followed by protein identification by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2019-11-07
AnnouncementXML	Submission_2019-11-06_23:38:08.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007085
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Koomen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-20 02:56:03	ID requested
⏵ 1	2019-11-06 23:42:52	announced

Ramello MC, Benza, ï, d I, Kuenzi BM, Lienlaf-Moreno M, Kandell WM, Santiago DN, Pab, ó, n-Salda, ñ, a M, Darville L, Fang B, Rix U, Yoder S, Berglund A, Koomen JM, Haura EB, Abate-Daga D, An immunoproteomic approach to characterize the CAR interactome and signalosome. Sci Signal, 12(568):(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Chimeric Antigen Receptors, Interactome, Tonic Signaling

Daniel Abate-Daga
contact affiliation	Moffitt Cancer Center
contact email	daniel.abatedaga@moffitt.org
lab head
John Koomen
contact affiliation	Moffitt Cancer Center
contact email	john.koomen@moffitt.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD007085

PRIDE project URI

• PRIDE
  1. PXD007085
     1. Label: PRIDE project
     2. Name: An immunoproteomic approach to characterize the CAR interactome and signalosome