PXD007078

PXD007078 is an original dataset announced via ProteomeXchange.

Title	MEIS homeodomain proteins facilitate PARP1/ARTD1-mediated eviction of histone H1
Description	Pre–B-cell leukemia homeobox (PBX) and myeloid ecotropic viral integration site (MEIS) proteins control cell fate decisions in many physiological and pathophysiological contexts, but how these proteins function mechanistically remains poorly defined. Focusing on the first hours of neuronal differentiation of adult subventricular zone–derived stem/progenitor cells, we describe a sequence of events by which PBX-MEIS facilitates chromatin accessibility of transcriptionally inactive genes: In undifferentiated cells, PBX1 is bound to the H1-compacted promoter/proximal enhancer of the neuron-specific gene doublecortin (Dcx). Once differentiation is induced, MEIS associates with chromatin-bound PBX1, recruits PARP1/ARTD1, and initiates PARP1-mediated eviction of H1 from the chromatin fiber. These results for the first time link MEIS proteins to PARP-regulated chromatin dynamics and provide a mechanistic basis to explain the profound cellular changes elicited by these proteins.
HostingRepository	PRIDE
AnnounceDate	2017-07-21
AnnouncementXML	Submission_2017-07-31_02:26:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-19 06:42:07	ID requested
1	2017-07-21 02:00:55	announced
⏵ 2	2017-07-31 02:26:59	announced	Updated publication reference for PubMed record(s): 28739678.

Hau AC, Grebbin BM, Agoston Z, Anders-Maurer M, M, ü, ller T, Gro, ß A, Kolb J, Langer JD, D, ö, ring C, Schulte D, MEIS homeodomain proteins facilitate PARP1/ARTD1-mediated eviction of histone H1. J Cell Biol, 216(9):2715-2729(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: MEIS, PARP1, histone H1

Julian David Langer
contact affiliation	Max Planck Institute of Biophysics, Frankfurt am Main, Germany
contact email	julian.langer@biophys.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD007078

PRIDE project URI

• PRIDE
  1. PXD007078
     1. Label: PRIDE project
     2. Name: MEIS homeodomain proteins facilitate PARP1/ARTD1-mediated eviction of histone H1