PXD007066

PXD007066 is an original dataset announced via ProteomeXchange.

Title	Proteomics Analysis of Human Breast Milk to Assess Breast Cancer Risk
Description	Detection of breast cancer (BC) in young women is challenging because mammography, the most common tool for detecting BC, is not effective on the dense breast tissue characteristic of young women. In addition to the limited means for detecting their BC, young women face a transient increased risk of pregnancy-associated BC. As a consequence, reproductively active women could benefit significantly from a tool that provides them with accurate risk assessment and early detection of BC. One potential method for detection of BC is biochemical monitoring of proteins and other molecules in bodily fluids such as serum, nipple aspirate, ductal lavage, tear, urine, saliva and breast milk. Of all these fluids, only breast milk provides access to a large volume of breast tissue, in the form of exfoliated epithelial cells, and to the local breast environment, in the form of molecules in the milk. Thus, analysis of breast milk is a non-invasive method with significant potential for assessing BC risk. Here we analyzed human breast milk by mass spectrometry (MS)-based proteomics to build a biomarker signature for early detection of BC. Ten milk samples from 8 women provided 5 paired-groups (cancer versus control) for analysis of differentially expressed proteins: 2 within woman comparisons (milk from a diseased breast versus a healthy breast of the same woman) and 3 across women comparisons (milk from a woman with cancer versus a woman without cancer). Despite a wide range in the time between milk donation and cancer diagnosis (cancer diagnosis occurred from 1 month before to 24 months after milk donation), the levels of some proteins differed significantly between cancer and control in several of the 5 comparison groups. These pilot data are supportive of the idea that molecular analysis of breast milk will identify proteins informative for early detection and accurate assessment of BC risk, and warrant further research.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:21.214.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roshanak Aslebagh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q-Tof Ultima

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-17 08:21:57	ID requested
1	2017-12-18 01:36:23	announced
⏵ 2	2024-10-22 04:39:29	announced	2024-10-22: Updated project metadata.

10.1002/elps.201700123;

Aslebagh R, Channaveerappa D, Arcaro KF, Darie CC, Proteomics analysis of human breast milk to assess breast cancer risk. Electrophoresis, 39(4):653-665(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Mass spectrometry,Breast cancer, Breast milk, Proteomics, Biomarkers

Costel C. Darie
contact affiliation	Clarkson University
contact email	cdarie@clarkson.edu
lab head
Roshanak Aslebagh
contact affiliation	Clarkson University
contact email	aslebar@clarkson.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD007066

PRIDE project URI

• PRIDE
  1. PXD007066
     1. Label: PRIDE project
     2. Name: Proteomics Analysis of Human Breast Milk to Assess Breast Cancer Risk