PXD007061

PXD007061 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Haloferax volcanii proteome turn-over analysis of a LonB mutant and co-IP results with anti-LonB
Description	The proteome turnover was examined in a conditional LonB mutant (HVLON3) of the model euryarchaeon H. volcanii under reduced (- trp) and nearly physiological (+ trp) LonB levels. HVLON3 was initially grown in minimal medium containing 14NH4Cl as nitrogen source in absence of trp and then switched to 15N-medium with/without trp (± Lon) to monitor 15N-label incorporation into newly synthesized proteins over time. In parallel, degradation of 14N-labeled proteins was estimated by comparing different time points of this culture via an internal standard grown on 13C-glucose. Protein samples (membrane and cytoplasm fractions) were processed by SDS-PAGE, digested with trypsin and analyzed by LC-MS/MS. Proteins were identified with Sequest and protein turnover, as well as statistical analysis, was achieved with the online platform QuPE. In addition, an in vivo crosslinking assay coupled to immunoprecipitation with anti-Lon antibody was performed in the H. volcanii H26 wt strain to detect interactions between LonB and its endogenous targets.
HostingRepository	jPOST
AnnounceDate	2018-02-19
AnnouncementXML	Submission_2022-09-18_04:00:24.921.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Micaela Cerletti
SpeciesList	scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; 2-pyrrolidone-5-carboxylic acid (Gln); unknown modification; deamidated residue
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-17 05:22:01	ID requested
1	2018-02-19 05:54:51	announced
2	2018-02-19 05:56:07	announced	Updated PubMed.
3	2018-09-19 00:17:09	announced	Updated CV, FTP location and Modification list.
4	2021-02-04 17:28:15	announced	2021-02-04: Updated FTP location.
⏵ 5	2022-09-18 04:00:25	announced	2022-09-18: Updated FTP location.

Publication List

Cerletti M, Paggi R, Troetschel C, Ferrari MC, Guevara CR, Albaum S, Poetsch A, De Castro R, LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. J Proteome Res, 17(3):1158-1171(2018) [pubmed]

Cerletti M, Paggi R, Troetschel C, Ferrari MC, Guevara CR, Albaum S, Poetsch A, De Castro R, LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. J Proteome Res, 17(3):1158-1171(2018) [pubmed]

Keyword List

submitter keyword: LonB protease, Haloferax volcanii, proteome turnover, IP, substrates

submitter keyword: LonB protease, Haloferax volcanii, proteome turnover, IP, substrates

Contact List

Rosana De Castro
lab head
Micaela Cerletti
contact affiliation	IIB-UNMdP
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000291/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000291/