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PXD007061

PXD007061 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHaloferax volcanii proteome turn-over analysis of a LonB mutant and co-IP results with anti-LonB
DescriptionThe proteome turnover was examined in a conditional LonB mutant (HVLON3) of the model euryarchaeon H. volcanii under reduced (- trp) and nearly physiological (+ trp) LonB levels. HVLON3 was initially grown in minimal medium containing 14NH4Cl as nitrogen source in absence of trp and then switched to 15N-medium with/without trp (± Lon) to monitor 15N-label incorporation into newly synthesized proteins over time. In parallel, degradation of 14N-labeled proteins was estimated by comparing different time points of this culture via an internal standard grown on 13C-glucose. Protein samples (membrane and cytoplasm fractions) were processed by SDS-PAGE, digested with trypsin and analyzed by LC-MS/MS. Proteins were identified with Sequest and protein turnover, as well as statistical analysis, was achieved with the online platform QuPE. In addition, an in vivo crosslinking assay coupled to immunoprecipitation with anti-Lon antibody was performed in the H. volcanii H26 wt strain to detect interactions between LonB and its endogenous targets.
HostingRepositoryjPOST
AnnounceDate2018-02-19
AnnouncementXMLSubmission_2022-09-18_04:00:24.921.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMicaela Cerletti
SpeciesList scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; 2-pyrrolidone-5-carboxylic acid (Gln); unknown modification; deamidated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-07-17 05:22:01ID requested
12018-02-19 05:54:51announced
22018-02-19 05:56:07announcedUpdated PubMed.
32018-09-19 00:17:09announcedUpdated CV, FTP location and Modification list.
42021-02-04 17:28:15announced2021-02-04: Updated FTP location.
52022-09-18 04:00:25announced2022-09-18: Updated FTP location.
Publication List
Cerletti M, Paggi R, Troetschel C, Ferrari MC, Guevara CR, Albaum S, Poetsch A, De Castro R, LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. J Proteome Res, 17(3):1158-1171(2018) [pubmed]
Keyword List
submitter keyword: LonB protease, Haloferax volcanii, proteome turnover, IP, substrates
Contact List
Rosana De Castro
lab head
Micaela Cerletti
contact affiliationIIB-UNMdP
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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