PXD007061
PXD007061 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Haloferax volcanii proteome turn-over analysis of a LonB mutant and co-IP results with anti-LonB |
Description | The proteome turnover was examined in a conditional LonB mutant (HVLON3) of the model euryarchaeon H. volcanii under reduced (- trp) and nearly physiological (+ trp) LonB levels. HVLON3 was initially grown in minimal medium containing 14NH4Cl as nitrogen source in absence of trp and then switched to 15N-medium with/without trp (± Lon) to monitor 15N-label incorporation into newly synthesized proteins over time. In parallel, degradation of 14N-labeled proteins was estimated by comparing different time points of this culture via an internal standard grown on 13C-glucose. Protein samples (membrane and cytoplasm fractions) were processed by SDS-PAGE, digested with trypsin and analyzed by LC-MS/MS. Proteins were identified with Sequest and protein turnover, as well as statistical analysis, was achieved with the online platform QuPE. In addition, an in vivo crosslinking assay coupled to immunoprecipitation with anti-Lon antibody was performed in the H. volcanii H26 wt strain to detect interactions between LonB and its endogenous targets. |
HostingRepository | jPOST |
AnnounceDate | 2018-02-19 |
AnnouncementXML | Submission_2022-09-18_04:00:24.921.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Micaela Cerletti |
SpeciesList | scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; 2-pyrrolidone-5-carboxylic acid (Gln); unknown modification; deamidated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-07-17 05:22:01 | ID requested | |
1 | 2018-02-19 05:54:51 | announced | |
2 | 2018-02-19 05:56:07 | announced | Updated PubMed. |
3 | 2018-09-19 00:17:09 | announced | Updated CV, FTP location and Modification list. |
4 | 2021-02-04 17:28:15 | announced | 2021-02-04: Updated FTP location. |
⏵ 5 | 2022-09-18 04:00:25 | announced | 2022-09-18: Updated FTP location. |
Publication List
Cerletti M, Paggi R, Troetschel C, Ferrari MC, Guevara CR, Albaum S, Poetsch A, De Castro R, LonB Protease Is a Novel Regulator of Carotenogenesis Controlling Degradation of Phytoene Synthase in Haloferax volcanii. J Proteome Res, 17(3):1158-1171(2018) [pubmed] |
Keyword List
submitter keyword: LonB protease, Haloferax volcanii, proteome turnover, IP, substrates |
Contact List
Rosana De Castro | |
---|---|
lab head | |
Micaela Cerletti | |
contact affiliation | IIB-UNMdP |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000291/ |